A study on genotoxicity of nanomaterials using mammalian cultured cells

利用哺乳动物培养细胞研究纳米材料的遗传毒性

• 批准号: 23710081
• 负责人: KAWANISHI Masanobu
• 金额: $ 2.83万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23710081/
• 关键词: ナノマテリアル; ナノマグネタイト; 遺伝毒性; 培養細胞; 小核試験; 姉妹染色分体交換試験; DNA二重鎖切断; 活性酸素種; 環境質定量化・予測; ナノ材料

We examined the genotoxicity of magnetite nanoparticles (primary particle size: 10 nm) on human A549 and Chinese hamster ovary (CHO) AA8 cells. Six hours’ treatment with the particles dose-dependently increased the frequency of micronuclei (MN) in the A549 and CHO AA8 cells up to 5.2% and 5.0% at a dose of 200 μg/mL (34 μg/cm2), respectively. In A549 cells, treatment with the nanoparticles (2 μg/mL) for 1 hour induced H2AX phosphorylation, which is suggestive of DNA double strand breaks (DSB). Treating CHO AA8 cells with 2 μg/mL magnetite for 1 hour resulted in a five times higher frequency of sister chromatid exchange (SCE) than the control level. We detected reactive oxygen species (ROS) in CHO cells treated with the particles. These findings indicate that magnetite nanoparticles induce ROS in mammalian cells, leading to the direct or indirect induction of DSB, followed by clastogenic events including MN and SCE.

我们检查了人A549和中国仓鼠卵巢（CHO）AA8细胞上磁铁矿纳米颗粒（初级粒径：10 nm）的遗传毒性。用颗粒剂量依赖的六个小时的治疗分别增加了A549中的微核（MN）的频率，而CHO AA8细胞分别在200μg/ml（34μg/cm2）的剂量分别高达5.2％和5.0％。在A549细胞中，用纳米颗粒（2μg/ml）处理1小时诱导H2AX磷酸化，这表明DNA双链断裂（DSB）。用2μg/ml磁铁矿处理1小时的CHO AA8细胞导致姊妹染色单体交换（SCE）的频率高五倍。我们在用颗粒处理的CHO细胞中检测到活性氧（ROS）。这些发现表明，磁铁矿纳米颗粒在哺乳动物细胞中诱导ROS，导致DSB的直接或间接诱导，然后是经典事件，包括MN和SCE。

项目成果

カオリンの遺伝毒性発現メカニズムの解明

高岭土遗传毒性表达机制的阐明

• 发表时间: 2012
• 作者: 池本実穂;川西優喜;戸塚ゆ加里,若林敬二;八木孝司
• 通讯作者: 八木孝司

Genotoxicity induced by nanoparticles

纳米颗粒诱导的遗传毒性

• 发表时间: 2011
• 作者: Yukari Totsuka;Tatsuya Kato;KousukeIshino;Shuichi Masuda;Dai Nakae;YukieTada;Masanobu Kawanishi;Takashi Yagi,Masatoshi Watanabe;Keiji Wakabayashi,Hitoshi Nakagama
• 通讯作者: Keiji Wakabayashi,Hitoshi Nakagama

Genotoxicity of multi-walled carbon nanotubes in both in vitro and in vivo assay systems

• DOI: 10.3109/17435390.2012.674571
• 发表时间: 2013-06-01
• 期刊: NANOTOXICOLOGY
• 影响因子: 5
• 作者: Kato, Tatsuya;Totsuka, Yukari;Wakabayashi, Keiji
• 通讯作者: Wakabayashi, Keiji

Genotoxicity and reactive oxygen species production induced by magnetite nanoparticles in mammalian cells

• DOI: 10.2131/jts.38.503
• 发表时间: 2013-06-01
• 期刊: JOURNAL OF TOXICOLOGICAL SCIENCES
• 影响因子: 2
• 作者: Kawanishi, Masanobu;Ogo, Sayaka;Yagi, Takashi
• 通讯作者: Yagi, Takashi

ナノ材料による小核誘導における相同組換え修復の関与

同源重组修复参与纳米材料微核诱导

• 发表时间: 2011
• 作者: 池本実穂;川西優喜,戸塚ゆ加里;若林敬二;八木孝司
• 通讯作者: 八木孝司