Response of intestinal macrophage to intestinal bacteria

肠道巨噬细胞对肠道细菌的反应

• 批准号: 23700917
• 负责人: NAKATA Kazue
• 金额: $ 2.75万
• 依托单位: Okayama Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23700917/
• 关键词: 腸管マクロファージ; 腸管感染症; 感染防御; 組織特異性; 腸管免疫機構; 腸内細菌; 腸管マクロファージ; 腸管感染症; 感染防御; 組織特異性; 腸管免疫機構; 腸内細菌

The study aimed to clarify the mechanism of foreign substances recognition and elimination by intestinal macrophages and to identify selective intestinal macrophage responses, such as phagocytosis, bactericidal activity, and cytokine production, induced by nonpathogenic E. coli, V. parahaemolyticus, S. aureus, and L. casei. The phagocytosis of L. casei by intestinal macrophages was significantly lower than that of E. coli, V. parahaemolyticus, S. aureus. The bactericidal activities of intestinal macrophages differed for different bacterial species. TNF-alpha and IL-12 production induced by E. coli, V. parahaemolyticus, and S. aureus stimulation was significantly higher than that induced by LPS but not L. casei. IL-10 production was same for LPS and all bacteria. The production of TNF-alpha and IL-12 in intestinal macrophages may be related to phagocytosis. These results confirmed that intestinal macrophages show different responses to individual bacteria specie.

该研究旨在阐明肠道巨噬细胞识别和消除异物的机制，并鉴定选择性肠道巨噬细胞反应，例如吞噬作用，杀菌活性和细胞因子的产生，由非人对病作用E. Coli诱导，V。parahaemolyticus，S。aureus，aureus和L. casi casi casi casi casei。肠道巨噬细胞对L. casei的吞噬作用明显低于大肠杆菌，V。parahayticus，S。金黄色葡萄球菌。不同细菌物种的肠道巨噬细胞的杀菌活性不同。大肠杆菌，副溶血杆菌和金黄色葡萄球菌刺激诱导的TNF-α和IL-12产生明显高于LPS诱导的LP，但不高于Casei。 LP和所有细菌的IL-10产生相同。肠道巨噬细胞中TNF-Alpha和IL-12的产生可能与吞噬作用有关。这些结果证实，肠道巨噬细胞对单个细菌规范的反应不同。