Corneal regeneration using epithelial, stromal, and endothelial precursor cells based on epigenetical control.

基于表观遗传控制，使用上皮、基质和内皮前体细胞进行角膜再生。

• 批准号: 23689072
• 负责人: MIMURA Tatsuya
• 金额: $ 17.47万
• 依托单位: Tokyo Women's Medical University (2012-2013)The University of Tokyo (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23689072/
• 关键词: 眼発生・再生医学; エピジェネティクス; 角膜再生; 角膜; メチル化; 幹細胞移植

We examined cellular aging and epigenetical alteration in corneal cells. We obtaiined corneal cells from endothelium, stoma, and epithelum of various aged donor corneas. Additionally, we obtained progenitor cells from each corneal cell layer by sphere-forming assay. By comparing the methylation among donor age, we found the ratio of methylation was higher in the cell from oleder donor cornea compared with younger donor conreas.

我们检查了角膜细胞中的细胞衰老和表观遗传改变。我们从各种老化的供体角膜的内皮，孔隙和上皮中获得角膜细胞。此外，我们通过形成球体的测定法从每个角膜细胞层获得了祖细胞。通过比较供体年龄之间的甲基化，我们发现OLEDER供体角膜细胞中甲基化的比率高于年轻的供体圆锥体。

项目成果

Influence of Axial Length on Conjunctivochalasis

• DOI: 10.1097/ico.0b013e318292b7a2
• 发表时间: 2013-08-01
• 期刊: CORNEA
• 影响因子: 2.8
• 作者: Mimura, Tatsuya;Yamagami, Satoru;Matsubara, Masao
• 通讯作者: Matsubara, Masao

Relation Between Total Tear IgE and Severity of Acute Seasonal Allergic Conjunctivitis

• DOI: 10.3109/02713683.2012.689069
• 发表时间: 2012-10-01
• 期刊: CURRENT EYE RESEARCH
• 影响因子: 2
• 作者: Mimura, Tatsuya;Usui, Tomohiko;Amano, Shiro
• 通讯作者: Amano, Shiro

Relationship Between Total Tear IgE and Specific Serum IgE in Autumnal Allergic Conjunctivitis

• DOI: 10.1097/ico.0b013e318253214b
• 发表时间: 2013-01-01
• 期刊: CORNEA
• 影响因子: 2.8
• 作者: Mimura, Tatsuya;Usui, Tomohiko;Amano, Shiro
• 通讯作者: Amano, Shiro

Aqueous Soluble Vascular Endothelial Growth Factor Receptor-2 in Macular Edema with Branch Retinal Vein Occlusion

• DOI: 10.3109/02713683.2013.821135
• 发表时间: 2013-12-01
• 期刊: CURRENT EYE RESEARCH
• 影响因子: 2
• 作者: Noma, Hidetaka;Mimura, Tatsuya
• 通讯作者: Mimura, Tatsuya

Corneal endothelial tissue bioengineering using cultured human corneal endothelial precursor cells(In : Jose A. Andrades, editor. Chapter 18, Regenerative Medicine and Tissue Engineering)(ISBN 978-953-51-1108-5)

使用培养的人角膜内皮前体细胞进行角膜内皮组织生物工程（见：Jose A. Andrades，编辑。第18章，再生医学和组织工程）（ISBN 978-953-51-1108-5）

• 作者: Mimura T;Yokoo S;Yamagami S
• 通讯作者: Yamagami S