Screening of low molecular compounds, which inhibit cell proliferation and survival

筛选抑制细胞增殖和存活的低分子化合物

• 批准号: 23659488
• 负责人: KANAKURA Yuzuru
• 金额: $ 2.33万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659488/
• 关键词: 低分子化合物; 増殖; 細胞周期; 細胞株; 細胞増殖; アポトーシス; スクリーニング; 増殖抑制

We tried to establish a new anti-proliferative drug through a following process: (1) screening of compounds to inhibit Baf3 cell growth (2) identification of target molecules for the selected compounds (3) analysis of physical effects of each possible compound. We screened approximately 10,000 low molecular compounds, which were provided from Tokyo University, and successfully isolated 61 compounds. Some inhibited Baf3 cell growth, or induced apoptosis, and the others induced morphological changes. Among them, we selected 4 compounds, T-167317, T-170633, T-005170, T-051702, which is water-soluble and has a specific structure. To identify their molecular targets, we just fixed the compounds onto FG beads.

我们试图通过以下过程来建立一种新的抗增殖药物：（1）筛选化合物以抑制BAF3细胞生长（2）鉴定所选化合物的靶分子（3）分析每种可能化合物的物理作用。我们筛选了大约10,000个低分子化合物，这些化合物是东京大学提供的，并成功隔离了61种化合物。一些抑制了BAF3细胞生长或诱导的凋亡，而另一些则诱导形态学变化。其中，我们选择了4种化合物T-167317，T-170633，T-005170，T-051702，它们是水溶性并且具有特定结构的。为了识别它们的分子靶标，我们只是将化合物固定在FG珠上。

项目成果

Advances in Hematopoietic Stem Cell Research

造血干细胞研究进展

• 发表时间: 2012
• 作者: Yokota T;Oritani K;Butz S;Ewers S;Vestweber D;Kanakura Y.
• 通讯作者: Kanakura Y.

Significance of novel HSC marker ESAM expression in cord blood

脐带血中新型 HSC 标志物 ESAM 表达的意义

• 作者: Fresquet V;et al.;T Ishibashi
• 通讯作者: T Ishibashi

Identification of osteoblast stimulating factor-5 as a novel regulator of early lymphocyte development.

鉴定成骨细胞刺激因子 5 作为早期淋巴细胞发育的新型调节剂。

• 发表时间: 2012
• 作者: Fujita N;et al
• 通讯作者: et al

Platelet-associated anti-GPIIb/IIIa autoantibodies in primary immune thrombocytopenia recognize highly restricted regions in the beta-propeller domain of GPIIb with clonality

原发性免疫性血小板减少症中血小板相关抗 GPIIb/IIIa 自身抗体可识别具有克隆性的 GPIIb β-螺旋桨结构域中高度受限的区域

• 作者: Kiyomizu K;et al
• 通讯作者: et al

Role of endothelial antigen ESAM in hematopoietic stem cells status

内皮抗原ESAM在造血干细胞状态中的作用

• 作者: Sudo T;et al
• 通讯作者: et al