Development of gas phase sterilization technology for nucleolytic degradation

溶核降解气相灭菌技术的开发

• 批准号: 23659331
• 负责人: OKAZAKI Toshihiko
• 金额: $ 2.41万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659331/
• 关键词: 産業衛生; 滅菌技術; 核酸; 気相滅菌機器; 新規滅菌器機開発; 核酸分解

We have improved air intake and exhaust system of gas generating apparatus equipped with autocatalytic reaction components, resulting in capable of generating stable concentration of sterilization gas in a warehouse (also capable of monitoring gas concentration), and developing down-sizing system of apparatus, and also developing small chamber system for evaluating each experimental parameters. (Patent application number: 2012-062880, registered as a trademark as Biovector[〇!R]: reference number 12T13SX01) By use of this small chamber of sterilization system, we have evaluated the effect of sterilization of biological indicators and resolution of nucleic acid molecule s (DNA, RNA). By the sterilization gas exposure for 60 minutes under the temperature of 37 to 50 ℃, the complete eradication effect in all the objective bacilli applied in the amount of bacilli of 106has been achieved. Also in the evaluation of nucleolytic degradation ability using Bioanalyzer, the complete degradation ef … More fect in both of double strand DNA (dsDNA) and single strand DNA (ssDNA) into 10 or less base pairs was elucidated by the time of exposure for 30 minutes of sterilization gas under the condition of both of moist and dry. It also revealed that nucleolytic degradation ability of Biovector[〇!R] is temperature dependent (50>45>37 ℃). In the examination using the real time-PCR method, the complete degradation effect in both of RNA and ssDNA have been achieved by the time of exposure for 15 minutes of sterilization gas. In evaluation by the mycoplasma bacillus, certain validity also has been show, and still need to be elucidated. Taken together, in this study, newly developed gaseous sterilization system revealed its nucleolytic degradation ability, which could not be attained in the conventional method. For the next step, we endeavor to develop this innovative sterilization technology into the application to medical device by way of biosafety evaluation including corrosiveness and persistence as well as biological toxicity. Less

我们改善了配备了自催化反应成分的气体产生设备的气体摄入量和排气系统，从而能够在仓库中产生稳定的灭菌气体浓度（也能够监测气体浓度），并开发出较小的设备系统，还可以开发小型腔室系统，以评估每个实验参数。 （专利申请编号：2012-062880，作为商标注册为生物驾驶仪[〇！r]：参考编号12T13SX01）通过使用这个小型灭菌系统，我们评估了生物学指标和核酸分子（DNA，RNA）分辨率的灭菌作用。通过在37至50°C的温度下，通过灭菌气体暴露60分钟，实现了106杆杆菌量应用的所有客观杆菌的完全放射效应。同样在使用生物分析仪评估核解溶解降解能力时，完全降解EF…在双链DNA（dsDNA）和单链DNA（ssDNA）中，在10分钟的暴露时间均在湿润和干燥的情况下，将三个链DNA（dsDNA）和单链DNA（ssDNA）均被暴露时间30分钟。它还表明，生物电动器[〇！r]的核降解能力是温度依赖性的（50> 45> 37℃）。在使用实时PCR方法的检查中，通过暴露于15分钟的灭菌气体时，已经达到了RNA和ssDNA的完全降解效应。在由支原体杆菌评估时，也已经显示出某些有效性，并且仍然需要阐明。综上所述，在这项研究中，新开发的气态灭菌系统揭示了其核降解能力，这在常规方法中无法实现。在下一步中，我们努力通过生物安全评估（包括腐蚀性和持久性以及生物毒性）将这种创新的灭菌技术开发到医疗设备的应用中。较少的

项目成果

細胞調製施設―運営・管理の課題と対策

细胞制备设施-运行管理问题及对策

• 作者: H. Madhyastha;M. Maruyama;K. Takamiya;岡崎利彦
• 通讯作者: 岡崎利彦

講演:「新規気相式核酸分解・滅菌装置を用いた災害現場に於ける衛生環境形成への応用」

讲座：“新型气相核酸分解/灭菌装置在灾害现场营造卫生环境的应用”

• 发表时间: 2012
• 作者: Ohnishi;S.;岡崎利彦
• 通讯作者: 岡崎利彦