Experimental approach for direct transformation of GABAergic neurons

GABA能神经元直接转化的实验方法

• 批准号: 23659092
• 负责人: SATO Makoto
• 金额: $ 2.41万
• 依托单位: University of Fukui
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659092/
• 关键词: 解剖学; 分化; 細胞; 組織; 神経科学; 細胞・組織; 脳・神経

（１） Cortical neurons that had been transfected with Ptf1a expressing vector migrated tangentially into the cortex, of which migratory behavior is typical for GABAergic neurons.（２）Ptf1a expression did not result in transformation of glutamatergic neurons into GABAergic neurons; no specific GABAergic neuronal markers were newly expressed in glutamatergic neurons by Ptf1a expression.（３） We collected RNAs from Ptf1a expressed neurons and searched for any Ptf1a-induced genes, then we identified several candidate genes.

（1）用PTF1A转染的皮质神经元在PTF1A中转染​​了皮质（2）PTF1A表达并未导致转化的伴侣神经元转化为GABA能神经元，并在谷氨酸氨前神经元中新表达。确定的严重性候选基因。

项目成果

Identification of long association neuron-specific genes in the mouse cerebral cortex.

小鼠大脑皮层长关联神经元特异性基因的鉴定。

• 作者: Oka;Y. et. al.
• 通讯作者: Y. et. al.

Molecular aspects shared by the cortical development and the spine formation, and their potential involvement in the network remodeling.

皮质发育和脊柱形成共有的分子方面，以及它们在网络重塑中的潜在参与。

• 发表时间: 2011
• 作者: Xie;M.-J. et al.;Toshimori K;佐藤 真
• 通讯作者: 佐藤 真

大脳皮質でのglia-guided locomotionの開始には、樹状突起の成長円錐が重要である

树突生长锥对于大脑皮层神经胶质引导的运动的启动很重要

• 发表时间: 2011
• 作者: 謝敏カク;他
• 通讯作者: 他

Post-natal treatment by a blood-brain-barrier permeable calpain inhibitor, SNJ1945 rescued defective neuronal migration and neuronal circuit formation in lissencephaly

产后通过血脑屏障渗透性钙蛋白酶抑制剂 SNJ1945 治疗可挽救无脑畸形的神经元迁移和神经元回路形成缺陷

• 发表时间: 2013
• 期刊: Sci. Rep
• 作者: Toba;S.;Tamura;Y.;Kumamoto;K.;Yamada;M.;Takao;K.;Hattori;S.;Miyakawa;T.;Kataoka;Y.;Azuma;M.;Hayasaka;K.;Amamoto;M.;Tominaga;K.;Wynshaw-Boris;A.;Wanibuchi;H.;Oka;Y.;Sato;M.;Kato;M. and Hirotsune;S
• 通讯作者: S

FILIP is involved in the morphological control of dendritic spine through actomyosin Regulation

FILIP 通过肌动球蛋白调节参与树突棘的形态控制

• 发表时间: 2012
• 作者: Hideshi Yagi;Kazuki Kuroda;Min-Jue Xie;Hiroshi Ikeda;Tokuichi Iguchi;Takashi Nagano;Munekazu Komada;Kazuyuki Murase;Masaru Okabe;Koichi Noguchi;Makoto Sato
• 通讯作者: Makoto Sato