Innate immunity involved in the onset of Henoch-Schoenlein purpura and nephritis

先天免疫参与过敏性紫癜和肾炎的发病

• 批准号: 23791183
• 负责人: ITO Naoko
• 金额: $ 2.5万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791183/
• 关键词: ヘノッホ・シェーンライン紫斑病; Toll-like receptor; A20; 血管炎; 腎炎; 紫斑病性腎炎; 自然免疫

We hypothesize that innate immunity may be involved in the onset of Henoch-Schoenlein purpura(HSP), and especially the variation of A20 expression, which is known as an inhibitor of NF-κB activation, can be the important factor for secondary nephritis and the aggravation of HSP. In this study, we analyzed the role of A20 for HSP by quantitative RT-PCR and flow cytometry of lymphocyte surface expression. The results demonstrated the differences between HSP patients and normal controls in A20 of the quantitative RT-PCR and PBMNCs expression after the stimulation of LPS, but no significant difference between HSP patients with and without nephritis. On the other hand, among the risk factors for nephritis, male and the patients with abdominal pain had high expression of quantitative RT-PCR of A20, which may imply that the variation of A20 expression may affect the onset of nephritis in HSP patients.

我们认为天然免疫可能与过敏性紫癜（HSP）的发病有关，尤其是作为NF-κB激活抑制剂的A20表达的变化可能是继发性肾炎和继发性肾炎的重要因素。本研究通过定量RT-PCR和流式细胞术检测淋巴细胞表面表达来分析A20对HSP的作用，结果显示了HSP患者与正常对照之间的差异。 LPS刺激后的定量RT-PCR和PBMCs表达在A20中，但HSP患者与无肾炎患者之间无显着性差异；另一方面，肾炎的危险因素中，男性和腹痛患者的发生率较高。定量 RT-PCR 检测 A20 的表达情况，提示 A20 表达的变化可能影响 HSP 患者肾炎的发病。