Cloning and Expression of Siderophore Biosynthetic Gene Clusters from Marine Metagenome

海洋宏基因组铁载体生物合成基因簇的克隆和表达

• 批准号: 23790134
• 负责人: FUJITA Masaki
• 金额: $ 2.83万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790134/
• 关键词: 天然物化学; 生合成; メタゲノム; 海洋天然物; 異宿主生産; シデロフォア

More than 50 siderophore producing clones were found from the marine metagenomic library. Among them, DNA sequences of the four clones were determined. By chemical and spectroscopic methods, vibrioferrin and bisucaberin were identified as active products from the obtained clones. Especially, isolation yield of vibrioferrin was 40 times higher than that of the original producer. The other clone had a biosynthetic gene cluster whose total size was about 25 kbp which is one of the largest one found by functional metagenomic screening. These results suggested the promising potential of the functional metagenomic technique for cloning of biosynthetic genes and heterologous production of active molecules.

从海洋宏基因组库中发现了50多个铁载体产生克隆。其中，确定了四个克隆的DNA序列。通过化学和光谱法，颤音蛋白和双核酸蛋白酶被确定为来自获得的克隆的活性产物。尤其是，颤音蛋白的隔离产率比原始生产商高40倍。另一个克隆具有生物合成基因簇，其总大小约为25 kbp，这是功能性元基因组筛选发现的最大的基因簇。这些结果表明，功能性宏基因组技术对生物合成基因克隆和活性分子的异源产生具有有希望的潜力。

项目成果

Phylogenetically Novel LuxI/LuxR-Type Quorum Sensing Systems Isolated Using a Metagenomic Approach

• DOI: 10.1128/aem.01442-12
• 发表时间: 2012-09
• 期刊: Applied and Environmental Microbiology
• 影响因子: 4.4
• 作者: E. Nasuno;N. Kimura;M. Fujita;C. Nakatsu;Y. Kamagata;S. Hanada

Heterologous Production of Siderophores by the Genes Cloned from a Marine Metagenome

从海洋宏基因组克隆的基因异源产生铁载体

• 发表时间: 2011
• 作者: 陶山正樹;奥田勝博;松本健司;新藤充;Masaki Fujita
• 通讯作者: Masaki Fujita

海洋メタゲノム由来シデロフォア生産クローンの解析

来自海洋宏基因组的铁载体产生克隆的分析

• 发表时间: 2012
• 作者: 藤野悠那・大塚雅巳・藤田雅紀

海洋細菌からの非環状ヒドロキサム酸型シデロフォアの単離と推定生合成遺伝子のクローニング

从海洋细菌中分离无环异羟肟酸铁载体并克隆假定的生物合成基因

• 发表时间: 2013
• 作者: 山口貴世志;池上恒雄;宮野悟;中村祐輔;古川洋一;藤田雅紀・酒井隆一
• 通讯作者: 藤田雅紀・酒井隆一

Cloning and Heterologous Expression of the Vibrioferrin Biosynthetic Gene Cluster from a Marine Metagenomic Library

• DOI: 10.1271/bbb.110379
• 发表时间: 2011-12-01
• 期刊: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
• 影响因子: 1.6
• 作者: Fujita, Masaki J.;Kimura, Nobutada;Otsuka, Masami
• 通讯作者: Otsuka, Masami