Roles of the Golgi apparatus in cell growth regulation

高尔基体在细胞生长调节中的作用

• 批准号: 18570173
• 负责人: NAKAMURA Nobuhiro
• 金额: $ 2.61万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18570173/
• 关键词: Golgi apparatus; cell cycle; signal transduction; maintenance of structure; phosphorvlation

GRASP65 is phosphorylated at S277 by cdc2/cyclin B and may participate in the regulation of mitotic entry. The role of GRASP65 S277 phosphorylation in cell cycle regulation was analyzed by two approaches. (1) Injection of recombinant GRASP65 G2A mutant, which cannot localize to the Golgi apparatus and accumulate in the cytoplasm, into the cells inhibited the phosphorylation of histone H3 in early prophase but not the centrosome separation. This result suggested that phosphorylation of histone H3 and centrosome separation are independently regulated at the onset of mitosis and S277 phosphorylation of GRASP65 may have some role in the regulation of those events. (2) Biochemical analyses revealed that Plk 1 binds to GRASP65 phosphorylated by mitotic e and this was dependent on the phosphorylation of S277. The binding is mediated by polo-box domain of Plk1. This result suggested that GRASP65 phosphorylated at S277 recruit Plk1 and function as a signaling scaffold at the onset of mitosis.

GRASP65 在 S277 处被 cdc2/cyclin B 磷酸化，可能参与有丝分裂进入的调节。通过两种方法分析了 GRASP65 S277 磷酸化在细胞周期调节中的作用。 (1)将不能定位于高尔基体并在细胞质中积累的重组GRASP65 G2A突变体注射到细胞中，抑制了前期早期组蛋白H3的磷酸化，但不抑制中心体分离。这一结果表明，组蛋白 H3 的磷酸化和中心体分离在有丝分裂开始时受到独立调节，GRASP65 的 S277 磷酸化可能在这些事件的调节中发挥一定作用。 (2)生化分析表明Plk 1与有丝分裂e磷酸化的GRASP65结合，这依赖于S277的磷酸化。该结合由 Plk1 的 polo-box 结构域介导。这一结果表明，GRASP65 在 S277 处磷酸化，募集 Plk1 并在有丝分裂开始时充当信号支架。

项目成果

The Interaction of Two Tethering Factors, p115 and COG complex, is Required for Golgi Integrity

高尔基体完整性需要两个束缚因子 p115 和 COG 复合物的相互作用

• 发表时间: 2007
• 作者: Sohda; M.; Misumi; Y.; Yoshimura; S.; Nakamura; N.; Fusano; T.; Ogata; S.; Sakisaka; S.; Ikehara; Y
• 通讯作者: Y

IntraGolgi distribution of the Conserved Oligomeric Golgi (COG) complex.

保守寡聚高尔基体 (COG) 复合体的高尔基内分布。

• 发表时间: 2006
• 作者: Vasile; E; et al.
• 通讯作者: et al.

Knockdown of Mitochondrial Heat Shock Protein 70 Promotes Progeria-like Phenotypes in Caenorhabditis elegans*

线粒体热休克蛋白 70 的敲低促进秀丽隐杆线虫的早衰样表型*

• DOI: 10.1074/jbc.m609025200
• 发表时间: 2007-02-23
• 期刊: Journal of Biological Chemistry
• 影响因子: 4.8
• 作者: K. Kimura;Nozomu Tanaka;N. Nakamura;S. Takano;S. Ohkuma
• 通讯作者: S. Ohkuma

Knockdown of mitochondrial heat shock protein 70 promotes progeria-like phenotypes in caenorhabditis elegans.

线粒体热休克蛋白 70 的敲低可促进秀丽隐杆线虫的早衰样表型。

• 发表时间: 2007
• 作者: Kimura; K.; et al.
• 通讯作者: et al.

The Interaction of Two Tethering Factors, p115 and COG complex, is Required for Golgi Integrity.

p115 和 COG 复合物这两个束缚因子的相互作用是高尔基体完整性所必需的。

• 发表时间: 2007
• 作者: Sohda; M.; et al.
• 通讯作者: et al.