Analysis of Cdx2 gene expression patterns and cell movement in mouse preimplantation embryos by live-cell imaging

通过活细胞成像分析小鼠植入前胚胎中的 Cdx2 基因表达模式和细胞运动

• 批准号: 23770259
• 负责人: TOYOOKA Yayoi
• 金额: $ 3万
• 依托单位: National Institute for Basic Biology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770259/
• 关键词: 細胞分化; 栄養外胚葉; 転写因子; Trophoblast

Establishment of Trophectoderm (TE) and inner cell mass (ICM) is the first cell lineage segregation in mouse development. Determination of the TE cell lineage is thought to be triggered by expression of Caudal-type transcriptional factor Cdx2 gene. To examine detailed expression patterns of Cdx2 and cell behavior during preimplantation development, we established Cdx2-EGFP reporter mice by which endogenous expression of Cdx2 can be visualized as EGFP fluorescence. We observed preimplantation embryos from Cdx2-GFP reporter mice by live-imaging, and found that cells internalized during molura stage once started to express Cdx2 when they were localized in outer position, then after internalization they participated in ICM and down-regulated Cdx2. These data suggest that these internalized cells can re-repress TE-specification pathway once activated, and behave as a part of pluripotent cell population.

滋养外胚层（TE）和内细胞团（ICM）的建立是小鼠发育中的第一次细胞谱系分离。 TE 细胞谱系的测定被认为是由尾型转录因子 Cdx2 基因的表达触发的。为了检查植入前发育过程中 Cdx2 的详细表达模式和细胞行为，我们建立了 Cdx2-EGFP 报告小鼠，通过该小鼠可以将 Cdx2 的内源表达可视化为 EGFP 荧光。我们通过活体成像观察了Cdx2-GFP报告小鼠的植入前胚胎，发现在molura阶段内化的细胞在位于外部位置时一度开始表达Cdx2，然后内化后它们参与ICM并下调Cdx2。这些数据表明，这些内化细胞一旦激活就可以重新抑制 TE 特异性途径，并表现为多能细胞群的一部分。

项目成果

Serial analysis of gene expression patterns and cell movement in mouse preimplantation embryos by Live-cell imaging.

通过活细胞成像对小鼠植入前胚胎中的基因表达模式和细胞运动进行系列分析。

• 发表时间: 2013
• 作者: Yayoi Toyooka;Sanae Oka;Toshihiko Fujimori
• 通讯作者: Toshihiko Fujimori

Serial analysis of gene expression patterns and cell movement in mouse preimplantation embryos by Live-cell imaging

通过活细胞成像对小鼠植入前胚胎中的基因表达模式和细胞运动进行系列分析

• 发表时间: 2013
• 作者: Yayoi Toyooka;Sanae Oka;Toshihiko Fujimori
• 通讯作者: Toshihiko Fujimori

Analysis of expression patterns of Cdx2 and cell movement in mouse preimplantation embryos by live-cell imaging

通过活细胞成像分析小鼠植入前胚胎中 Cdx2 的表达模式和细胞运动

• 发表时间: 2014
• 作者: Yayoi Toyooka;Sanae Oka;Toshihiko Fujimori
• 通讯作者: Toshihiko Fujimori