Role of FBP17,an EFC/F-BAR protein, in phagocytic cupformation

FBP17（一种 EFC/F-BAR 蛋白）在吞噬细胞形成中的作用

• 批准号: 23770228
• 负责人: TSUJITA Kazuya
• 金额: $ 2.91万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770228/
• 关键词: F-BAR; ドメイン; アクチン重合; F-BAR ドメイン; 細胞膜; F-BAR domain; アクチン細胞骨格; phagocytosis

By performing in vitro analysis, we directly showed the mechanism by which FBP17 regulates actin polymerization on the membrane tubules. Physiologically, it was found that FBP17 is recruited to phagocytic cup via its F-BAR domain and that this accumulation is critical for phagocytic cup formation. Furthermore, RNAi and its rescue experiments revealed that the assembly of FBP17 at the plasma membrane is dependent on its F-BAR domain, promoting actin polymerization at phagocytic cup membrane. These data suggest that invaginated membrane caused by FBP17 may function as a scaffold for actin polymerization.

通过体外分析，我们直接揭示了FBP17调节膜小管肌动蛋白聚合的机制。生理学上，发现 FBP17 通过其 F-BAR 结构域被招募到吞噬杯，并且这种积累对于吞噬杯的形成至关重要。此外，RNAi及其救援实验表明，FBP17在质膜上的组装依赖于其F-BAR结构域，促进吞噬杯膜上的肌动蛋白聚合。这些数据表明，FBP17 引起的内陷膜可能充当肌动蛋白聚合的支架。

项目成果

Characterization of the EFC/F-BAR domain protein

EFC/F-BAR 结构域蛋白的表征

• 发表时间: 2011
• 期刊: CHO2.Genes to Cell
• 作者: Uezu A;Umeda K;Tsujita K;Suetsugu S;Takenawa T;Nakanishi H
• 通讯作者: Nakanishi H

Regulation of membrane mediated actin assembly by EFC/F-BAR proteins.

EFC/F-BAR 蛋白调节膜介导的肌动蛋白组装。

• 发表时间: 2011
• 作者: 辻田 和也

SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide-binding domain

• DOI: 10.1083/jcb.201012161
• 发表时间: 2011-05-30
• 期刊: JOURNAL OF CELL BIOLOGY
• 影响因子: 7.8
• 作者: Hasegawa, Junya;Tokuda, Emi;Itoh, Toshiki
• 通讯作者: Itoh, Toshiki

Physicochemical analysis from real-time imaging of liposome tubulation reveals the characteristics of individual F-BAR domain proteins.

• DOI: 10.1021/la303902q
• 发表时间: 2013-01
• 期刊: Langmuir : the ACS journal of surfaces and colloids
• 作者: Y. Tanaka-Takiguchi;T. Itoh;K. Tsujita;Shunsuke Yamada;M. Yanagisawa;Kei Fujiwara;Akihisa Yamamoto;M. Ichikawa;K. Takiguchi

Antagonistic regulation of F-BAR protein assemblies controls actin polymerization during podosome formation

• DOI: 10.1242/jcs.122515
• 发表时间: 2013-05
• 期刊: Journal of Cell Science
• 影响因子: 4
• 作者: K. Tsujita;A. Kondo;S. Kurisu;Junya Hasegawa;T. Itoh;T. Takenawa