Structural study on the generation mechanism of nitrous oxide by integral membrane protein nitric oxide reductase

整合膜蛋白一氧化氮还原酶生成一氧化二氮机制的结构研究

• 批准号: 23770144
• 负责人: HINO Tomoya
• 金额: $ 3万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770144/
• 关键词: 金属タンパク質; 膜蛋白質; 一酸化窒素還元酵素; 亜酸化窒素; 呼吸酵素; 抗体; 膜タンパク質; X線結晶構造解析; 分子進化; 金属タンパク質; 膜蛋白質; 一酸化窒素還元酵素; 亜酸化窒素; 呼吸酵素; 抗体; 膜タンパク質; X線結晶構造解析; 分子進化

Bacterial nitric oxide reductase is a membrane integral enzyme that catalyzes the reduction of nitric oxide to nitrous oxide during the nitrate respiration, an anaerobic respiration of facultative anaerobic organisms. In the present study, I determined the crystal structure of nitric oxide reductase from Pseudomonas aeruginosa in the fully reduced state and revealed that no obvious structural changes were induced including active site upon reduction of all metal sites

细菌一氧化氮还原酶是一种膜积分酶，可在硝酸盐呼吸过程中催化一氧化氮向一氧化二氮的还原，这是辅助厌氧生物的厌氧呼吸。在本研究中，我确定了完全还原状态下的铜绿假单胞菌的一氧化氮还原酶的晶体结构