How do SUMO regulate cell cycle progression in Arabidopsis?

SUMO 如何调节拟南芥细胞周期进程？

• 批准号: 23770053
• 负责人: ISHIDA Takashi
• 金额: $ 2.91万
• 依托单位: Kumamoto University (2013)Nara Institute of Science and Technology (2012)The Institute of Physical and Chemical Research (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770053/
• 关键词: SUMO; 細胞周期; シロイヌナズナ; 翻訳後修飾; 「国際研究者交流」; オランダ王国; 核内倍加; SUMO; 細胞周期; シロイヌナズナ; 翻訳後修飾; 「国際研究者交流」; オランダ王国; 核内倍加

Scrupulously designed cell cycle regulation is the fundamental mechanism for development of higher organisms. SUMO-mediated posttranslational modification appeared to be involved in the developmental regulation since the fact that a mutation in SUMO E3 ligase HPY2 affect cell cycle progression have reported in Arabidopsis. However, further analyses are needed to understand the molecular mechanism. In this study, we analyzed cell cycle regulators, e.g. CDKs, AURs and RBR on the SUMO-mediated cell cycle regulation context. We established experimental conditions to express and purify these proteins. Our semi-in vitro SUMOylation assays showed that the cell cycle regulators are SUMOylated proteins. Then we identified SUMOylation sites for the substrates. Furthermore, we generated binary vectors harboring SUMO-null version of the regulators and introduced into their mutants plants. The mutant version lose their functions, suggesting that the SUMOylation in essential for their functions.

精心设计的细胞周期调节是发展较高生物体的基本机制。 Sumo介导的翻译后修饰似乎参与了发育调控，因为Sumo E3连接酶HPY2中的突变会在拟南芥中报道了细胞周期的进展。但是，需要进一步的分析来了解分子机制。在这项研究中，我们分析了细胞周期调节剂，例如Sumo介导的细胞周期调节环境上的CDK，AURS和RBR。我们建立了表达和净化这些蛋白质的实验条件。我们的半体外Sumoylation分析表明，细胞周期调节剂是Sumoypated蛋白。然后，我们确定了底物的sumoylation位点。此外，我们生成了带有Sumo-Null版本的调节器并引入其突变体植物的二元载体。突变版本失去了功能，表明其功能所必需的sumoylation。