Mechanism of structural maintenance of mammalian rDNA cluster

哺乳动物rDNA簇结构维持机制

• 批准号: 23770009
• 负责人: AKAMATSU Yufuko
• 金额: $ 2.91万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770009/
• 关键词: rDNA; DNA複製; 複製フォーク進行阻害; ゲノム再編成; リボソームRNA遺伝子; DNA複製阻害

To understand molecular mechanism maintaining mammalian rDNA cluster, DNA replication fork barrier (RFB) at 3' end of rRNA coding region was investigated. Using two -dimensional gel electrophoresis method, DNA replication fork movement was visualized. In mammalian cells, multiple RFB signals were detected and a part of them, as previously reported, were TTF1 dependent to block movement of replication fork. The other RFB, however, did not involve TTF-1, rather previously unknown mechanism functions. These results imply that collision between replication fork and rRNA transcription is prevented through multiple pathways in mammalian rDNA cluster.

为了了解维持哺乳动物rDNA簇的分子机制，研究了RRNA编码区域3'末端的DNA复制叉屏障（RFB）。使用两种维凝胶电泳方法，可以看到DNA复制叉运动。在哺乳动物细胞中，检测到多个RFB信号，如先前报道的一部分是TTF1取决于阻断复制叉的运动。但是，其他RFB不涉及TTF-1，而是以前未知的机制功能。这些结果表明，复制叉和rRNA转录之间的碰撞是通过哺乳动物rDNA簇中的多个途径阻止的。

项目成果

Rad51 presynaptic filament stabilization function of the mouse Swi5-Sfr1 heterodimeric complex.

• DOI: 10.1093/nar/gks305
• 发表时间: 2012-08
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Tsai SP;Su GC;Lin SW;Chung CI;Xue X;Dunlop MH;Akamatsu Y;Jasin M;Sung P;Chi P
• 通讯作者: Chi P

Characterization of the mammalian rDNA replication fork barrier

哺乳动物 rDNA 复制叉屏障的表征

• 发表时间: 2012
• 作者: Shinya Takahata;Yaxin Yu;Yota Murakami;David J. Stillman;Yufuko Akamatsu
• 通讯作者: Yufuko Akamatsu