Innovative regenerative medicine of hearing loss after cochlear ischemia by embryonic stem cells

胚胎干细胞治疗耳蜗缺血后听力损失的创新再生医学

• 批准号: 23791903
• 负责人: TAKAGI Taro
• 金额: $ 2.83万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791903/
• 关键词: 内耳再生; ES細胞; 突発性難聴; 虚血性内耳障害; 骨髄幹細胞; 静脈内投与; 神経前駆細胞; 再生療法

We investigated the influence of HSCs on progressive hair cell degeneration after transient cochlear ischemia in gerbils. Transient cochlear ischemia was produced by extracranial occlusion of the bilateral vertebral arteries. HSCs were administrated via femoral vein. Transplanted cells were confirmed in the spiral modiolar artery but was not found in the organ of Corti. Sequential changes in hearing were assessed by ABR. HSCs group was significantly decreased ABR threshold shift compared to the control group. The average ratio of dead/intact cells of inner hair cells was significantly smaller in the HSCs group than control group. The increasing of trophic factors protein level in the organ of Corti in the HSCs group indicated that HSCs upregulated the trophic factors. The intravenous injected HSCs could prevent hair cell degeneration and ameliorate hearing impairment. These results suggested that HSCs had therapeutic potential possibly through paracrine effects.

我们研究了瞬时人耳蜗缺血后，HSC对Gerbils瞬时毛细胞变性的影响。瞬态耳蜗缺血是通过双侧椎动脉的颅外阻塞产生的。 HSC通过股静脉管理。在螺旋模质动脉中证实了移植的细胞，但在Corti器官中未发现。听力的顺序变化通过ABR评估。与对照组相比，HSC组的ABR阈值移位显着降低。在HSC组中，内毛细胞的死亡/完整细胞的平均比率明显小于对照组。 HSC组中Corti器官中蛋白质水平的营养因子水平的增加表明，HSC上调了营养因子。静脉注射的HSC可以防止毛细胞变性并改善听力障碍。这些结果表明，HSC具有通过旁分泌作用具有治疗潜力。

项目成果

虚血性内耳障害に対する造血幹細胞静脈内投与の効果

静脉注射造血干细胞对缺血性内耳疾病的影响

• 发表时间: 2013
• 作者: 高木太郎;吉田正;白馬伸洋;暁清文
• 通讯作者: 暁清文

Hematopoietic stem cells prevent hair cell death after transient cochlear ischemia

造血干细胞可防止短暂性耳蜗缺血后毛细胞死亡

• 发表时间: 2013
• 作者: Taro Takagi;Tadashi yoshida;Nobuhiro Hakuba;Naohito Hato;Kiyofumi Gyo
• 通讯作者: Kiyofumi Gyo

愛媛大学での基礎および臨床研究の現状

爱媛大学基础与临床研究现状

• 发表时间: 2012
• 作者: Taro Takagi;Tadashi yoshida;Nobuhiro Hakuba;Naohito Hato;Kiyofumi Gyo;髙木太郎;髙木太郎
• 通讯作者: 髙木太郎

骨髄幹細胞投与による虚血性内耳障害の治療

通过给予骨髓干细胞治疗缺血性内耳疾病

• 发表时间: 2013
• 作者: Taro Takagi;Tadashi yoshida;Nobuhiro Hakuba;Naohito Hato;Kiyofumi Gyo;髙木太郎
• 通讯作者: 髙木太郎