The drug-resistant mechanism and rapid detection of the Pseudomonas aeruginosa derived from urinary tract infection

尿路感染铜绿假单胞菌的耐药机制及快速检测

• 批准号: 23791756
• 负责人: MATSUMOTO Minori
• 金额: $ 2.08万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791756/
• 关键词: 緑膿菌; 抗菌薬耐性; 迅速診断法; 薬剤耐性

Pseudomonas aeruginosa has some mechanisms of multi-drug resistance, including efflux pump system and amino acid mutation related to the drug resistance. We investigate the molecular biological mechanism of resistance to antibiotics, and seek for the rapid detection of the resistance. A hundred and fourteen strains of P.aeruginosa isolated from UTI patients were used in this study. Genomic DNA fromeach strain was subjected to PCR amplification of 225 bp in gyrA and 166 bp in ParC spanning QRDRs. After we performed DNA sequencing of these ampliconsand identification of mutations in the QRDRs, DHPLC was undertaken to investigate whether its results correlate the distinctive chromatogram with their DNA mutations pattern. In terms of efflux pump system in Pseudomonas aeruginosa, I examined the evaluation of the RNA frequency in the representative protein (mexB, mexC, mexE, mexY, muxA) related to efflux pump and the relationship with the drug resistance using technique of the quantitative real-time PCR.

铜绿假单胞菌具有多种耐药机制，包括外排泵系统和与耐药相关的氨基酸突变等。我们研究抗生素耐药性的分子生物学机制，并寻求耐药性的快速检测。本研究使用了从 UTI 患者中分离出的 114 株铜绿假单胞菌。每个菌株的基因组 DNA 在 gyrA 中进行 225 bp 的 PCR 扩增，在 ParC 中进行 166 bp 的 PCR 扩增，跨越 QRDR。在我们对这些扩增子进行 DNA 测序并鉴定 QRDR 中的突变后，我们进行了 DHPLC，以研究其结果是否将独特的色谱图与其 DNA 突变模式相关联。就铜绿假单胞菌的外排泵系统而言，我使用定量真实技术研究了与外排泵相关的代表性蛋白质（mexB、mexC、mexE、mexY、muxA）中RNA频率的评估及其与耐药性的关系。时PCR。

项目成果

尿路感染症由来緑膿菌臨床株における高速液体クロマトグラフィー法によるフルオロキノロン系抗菌薬耐性の迅速診断法確立への検討

高效液相色谱法快速诊断尿路感染铜绿假单胞菌临床菌株氟喹诺酮类抗菌药物耐药性的研究

• 发表时间: 2011

Rapid detection of the gyrA and parC mutations in fluoroquinolone-resistant Pseudomonas aeruginosa strain by denaturing high-performance liquid chromatography in UTI patients in Japan

通过变性高效液相色谱快速检测日本 UTI 患者中耐氟喹诺酮类铜绿假单胞菌菌株的 gyrA 和 parC 突变

• 发表时间: 2013