The supercooling technology in preservation of organ grafts

过冷技术在器官移植保存中的应用

• 批准号: 23791511
• 负责人: WATANABE Toshihiko
• 金额: $ 2.75万
• 依托单位: National Research Institute for Child Health and Development
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791511/
• 关键词: 過冷却凍結; 臓器保存; 臓器移植; 心移植; 脳死移植; 磁場環境下凍結; マウス

Current medical transplantation confronts major problems such as the shortage of donors and geographical restrictions that inhibit efficient utilization of finite donor organs. We investigated whether a strategy which best preserves organ grafts can be achieved by the use of a novel refrigerating chamber, which is capable of establishing a supercooled and unfrozen state stably by generating an electrostatic field in its inside. When mice heart was stored in the supercooled conditions, the levels of major biochemical markers leaked from the preserved organs were significantly lower than in the ordinary hypothermic storage. No apparent tissue damages were observed histologically after the supercooled preservation. After heart transplantation, rate of re-heart beating in supercooled condition was significantly higher. Our results suggest that the use of this supercooling refrigerator improves organ preservation and may provide an innovative technique for human organ transplantation.

当前的医疗移植面临着主要问题，例如捐助者的短缺和抑制有限供体器官有效利用的地理限制。我们研究了通过使用新型冷藏室可以实现最能保留器官移植的策略，该室能够通过在其内部产生静电场来建立超冷和未脱落的状态。当将小鼠心脏存储在超冷条件下时，从保存的器官中泄漏的主要生化标记水平显着低于普通的低温储存。超冷保存后，没有观察到组织学明显的组织损伤。心脏移植后，在超冷条件下重新心脏跳动的速度明显更高。我们的结果表明，这种超冷冰箱的使用改善了器官的保存，并可能为人体器官移植提供创新技术。

项目成果

Congenital pouch colonの1例

先天性袋状结肠的一个例子

• 发表时间: 2013
• 作者: 渡辺稔彦;大野通暢;渕本康史
• 通讯作者: 渕本康史

Congenital median raphe cysts : Coexistence of cystic lesions and canal like lesions

先天性中缝囊肿：囊性病变和管样病变并存

• 发表时间: 2013
• 期刊: Open J Pediatr 2013
• 作者: Takahashi M;Watanabe T;Sato K;Ohno M;Yamada K;Takezoe T;Fuchimoto Y;Ohkita H;Matsuoka K;Kanamori Y.
• 通讯作者: Kanamori Y.

Primary great saphenous vein aneurysm in a five-year-old boy

五岁男童原发性大隐静脉动脉瘤

• 发表时间: 2014
• 期刊: J Pediatr Surg Case Report 2014
• 作者: Takahiro Shimizu;Toshihiko Watanabe;Kaori Sato;Michinobu Ohno;Masataka Takahashi;Toshiko Takezoe;Yasushi Fuchimoto;Yutaka Kanamori
• 通讯作者: Yutaka Kanamori

IFALDを呈する新生児外科疾患に対するFish oilの役割(シンポジウム1小児消化器疾患の栄養管理)

鱼油在以 IFALD 为表现的新生儿外科疾病中的作用（研讨会 1 小儿胃肠道疾病的营养管理）

• 发表时间: 2013
• 作者: 渡辺稔彦;山田耕嗣;山田和歌;髙橋正貴;大野通暢;佐藤かおり;田中秀明;渕本康史;伊藤玲子;新井勝大;金森豊
• 通讯作者: 金森豊

oncologic emergencyを呈した縦隔ラブドイド腫瘍の1例

因肿瘤急症而出现的纵隔横纹肌样瘤一例

• 发表时间: 2013
• 作者: 渡辺稔彦;清水隆弘;右田美里;竹添豊志子;髙橋正貴;佐藤かおり;大野通暢;清谷知賀子;森鉄也;松本公一;渕本康史;金森豊
• 通讯作者: 金森豊