Electrochemical regulation of nucleic acids hybridization

核酸杂交的电化学调控

• 批准号: 23655068
• 负责人: IHARA Toshihiro
• 金额: $ 2.5万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23655068/
• 关键词: 生体分析; 人工核酸; 電気化学; 電極修飾; DNAセンサー; 電気化学的制御; DNAコンジュゲート; 核酸; 分子認識; センサー; フェロセン; 金属錯体; ターピリジン; BNA; LNA; 協同性; 錯生成; ハイブリダイゼーション; 生体分析; 人工核酸; 電気化学; 電極修飾; DNAセンサー; 電気化学的制御; DNAコンジュゲート; 核酸; 分子認識; センサー; フェロセン; 金属錯体; ターピリジン; BNA; LNA; 協同性; 錯生成; ハイブリダイゼーション

I have been trying to immobilize modified DNA carrying disulfide-bridged deoxyribose (ssDNA) and two terpyridine units (terpy2DNA) on carbon and gold electrode, respectively. I expect that I can monitor the hybridization by the change in their electrochemical properties.ssDNA was immobilized on HPOG electrode through stacking interaction of aromatic molecules such as pyrene and perylene onto the HOPG. terpy2DNA was immobilized on Au electrode by using specific affinity of sulfhydryl group, which was modified at the end of terpy2DNA, to Au. Unfortunately, the electrochemical signal of sulfhydryl/disulfide redox pair in ssDNA was not observed. The potential would be out of the measurement window. terpy2DNA formed intramolecular 1:2 complex with some divalent transition metal ions to form [Fe(terpy)2]2+. The redox profile of [Fe(terpy)2]2+/3+ on terpy2DNA was clearly observed on the Au electrode. The hybridization of terpy2DNA was monitored by frequency dependence on SWV measurement.

我一直在试图将携带二硫键桥的脱氧核糖（SSDNA）和两个terpyridine单位（Terpy2DNA）分别固定在碳和金电极上。我希望我可以通过其电化学性质的变化来监测杂交。SSDNA通过将芳香族分子（例如pyrene和perylene）的堆叠相互作用固定在HPOG电极上。通过使用在Terpy2DNA末端修饰的硫赖莱基团的特异性亲和力将Terpy2DNA固定在AU电极上，并将其固定在AU上。不幸的是，未观察到ssDNA中亚硫酸/二硫化物氧化还原对的电化学信号。潜力将超出测量窗口。 Terpy2DNA形成分子内1：2复合物，具有一些二价过渡金属离子，形成[Fe（Terpy）2] 2+。在Au电极上清楚地观察到了Terpy2DNA上[Fe（Terpy）2] 2+/3+的氧化还原轮廓。 Terpy2DNA的杂交通过频率依赖性对SWV测量进行监测。