Electrochemical regulation of nucleic acids hybridization

核酸杂交的电化学调控

• 批准号: 23655068
• 负责人: IHARA Toshihiro
• 金额: $ 2.5万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23655068/
• 关键词: 生体分析; 人工核酸; 電気化学; 電極修飾; DNAセンサー; 電気化学的制御; DNAコンジュゲート; 核酸; 分子認識; センサー; フェロセン; 金属錯体; ターピリジン; BNA; LNA; 協同性; 錯生成; ハイブリダイゼーション

I have been trying to immobilize modified DNA carrying disulfide-bridged deoxyribose (ssDNA) and two terpyridine units (terpy2DNA) on carbon and gold electrode, respectively. I expect that I can monitor the hybridization by the change in their electrochemical properties.ssDNA was immobilized on HPOG electrode through stacking interaction of aromatic molecules such as pyrene and perylene onto the HOPG. terpy2DNA was immobilized on Au electrode by using specific affinity of sulfhydryl group, which was modified at the end of terpy2DNA, to Au. Unfortunately, the electrochemical signal of sulfhydryl/disulfide redox pair in ssDNA was not observed. The potential would be out of the measurement window. terpy2DNA formed intramolecular 1:2 complex with some divalent transition metal ions to form [Fe(terpy)2]2+. The redox profile of [Fe(terpy)2]2+/3+ on terpy2DNA was clearly observed on the Au electrode. The hybridization of terpy2DNA was monitored by frequency dependence on SWV measurement.

我一直在尝试将携带二硫桥脱氧核糖（ssDNA）和两个三联吡啶单元（terpy2DNA）的修饰DNA分别固定在碳电极和金电极上。我希望我可以通过电化学性质的变化来监测杂交。通过芘和苝等芳香族分子在HOPG上的堆积相互作用，将ssDNA固定在HPOG电极上。利用terpy2DNA末端修饰的巯基对Au的特异性亲和力将terpy2DNA固定在Au电极上。不幸的是，没有观察到单链DNA中巯基/二硫键氧化还原对的电化学信号。电势将超出测量窗口。 terpy2DNA与一些二价过渡金属离子形成分子内1:2复合物，形成[Fe(terpy)2]2+。在 Au 电极上可以清楚地观察到 terpy2DNA 上的 [Fe(terpy)2]2+/3+ 的氧化还原曲线。通过对 SWV 测量的频率依赖性来监测 terpy2DNA 的杂交。

项目成果

Photochemically relevant DNA-based molecular systems enabling chemical and signal transductions and their analytical applications

• DOI: 10.1016/j.jphotochemrev.2012.03.002
• 发表时间: 2012-06
• 期刊: Journal of Photochemistry and Photobiology C-photochemistry Reviews
• 影响因子: 13.6
• 作者: T. Ihara;Yusuke Kitamura

"金属錯体の特異的な形成および相互作用を利用した新規核酸プローブの開発", "Development of Novel Nucleic Acid Probes Based on the Template-directed Formation of Metal Complexes"

“基于模板定向形成金属配合物的新型核酸探针的开发”

• 发表时间: 2013
• 作者: 北村裕介;井原敏博
• 通讯作者: 井原敏博

Metallo-regulation of the Bimolecular Triplex Formation of a Peptide

肽双分子三链体形成的金属调控

• 发表时间: 2013
• 期刊: Nucleic Acid Dalton Trans
• 作者: H. Shimada;T. Sakurai;Y. Kitamura;H. Matsuura;T. Ihara
• 通讯作者: T. Ihara

Ion-directed Drastic Conformational Change of DNA and Its Application to DNAzyme Activity Control

离子引导DNA的剧烈构象变化及其在DNAzyme活性控制中的应用

• 发表时间: 2013
• 作者: H. Ohura;T. Furuzono;C. Shirahama;Y. Kitamura;T. Ihara
• 通讯作者: T. Ihara

Versatile Molecular Devices Based on the Reversible Formation of Luminous Lanthanide Complexes

基于发光镧系元素配合物可逆形成的多功能分子器件

• 发表时间: 2012
• 作者: Y. Kitamura;S. Yamamoto;Y. Osawa;T. Ihara
• 通讯作者: T. Ihara