Innovative improvement of cancer therapeutic adenovirus vector carrying REIC gene

携带REIC基因的癌症治疗腺病毒载体的创新改进

• 批准号: 23650625
• 负责人: HUH Namho
• 金额: $ 2.33万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23650625/
• 关键词: がん遺伝子治療; アデノウイルス; REIC; DKK3; REIC/Dkk-3; がん; 遺伝子治療

Adenovirus vector is one of the most widely used vectors for cancer gene therapies. One of the serious problems associated with adenovirus vector-based gene therapy is that expression of Coxsackievirus and Adenovirus Receptor (CAR), a receptor for the adenovirus vector, is often reduced among advanced cancers. In this study, we attempted to highly enhance expression of a cargo gene by modifying promoter and applying a developed adenovirus-adaptor protein. A newly designed Ad-REIC showed 10 to 100 fold higher expression in a cancer specific manner than that attained by previous one. This vector certainly improved the therapeutic effect for a wide variety of human cancers. The selective anti-cancer function of the new Ad-REIC shows a great promise for clinical application.

腺病毒载体是用于癌症基因疗法的最广泛使用的载体之一。与基于腺病毒病毒载体的基因疗法相关的严重问题之一是，腺病毒载体的受体Coxsackievievivirus和腺病毒受体（CAR）通常会降低晚期癌症的表达。在这项研究中，我们试图通过修饰启动子并应用开发的腺病毒 - 适应器蛋白来高度增强货物基因的表达。新设计的AD-REIC以癌症特异性的方式显示出10至100倍的表达，比以前的表达方式更高。该矢量无疑改善了各种人类癌症的治疗作用。新AD-REIC的选择性抗癌功能显示出对临床应用的巨大希望。

项目成果

A new cytosolic pathway from a Parkinson's disease-associated kinase, BRPK/PINK1: activation of AKT via MTORC2

帕金森病相关激酶 BRPK/PINK1 的新胞质途径：通过 MTORC2 激活 AKT

• 发表时间: 2011
• 期刊: J Biol Chem
• 影响因子: 4.8
• 作者: Murata H;Sakaguchi M;Jin Y;Sakaguchi Y;Futami JI;Yamada H;Kataoka K;Huh NH
• 通讯作者: Huh NH

TRAF6 Ubiquitinates and Stabilizes PINK1 on Damaged Mitochondria Through Interaction with SARM1

TRAF6 通过与 SARM1 相互作用泛素化并稳定受损线粒体上的 PINK1

• 发表时间: 2012
• 作者: 村田 等

REIC/Dkk-3 の扁平上皮における発現とその制御因子の探索

REIC/Dkk-3在鳞状上皮中的表达及其调控因子的寻找

• 作者: 片岡 健;阪口政清;杜 剛;前原奈都美;村田 等;許 南浩
• 通讯作者: 許 南浩

REIC/Dkk-3-encoding adenoviral vector as a potentially effective therapeutic agent for bladder cancer

• DOI: 10.3892/ijo.2012.1503
• 发表时间: 2012-08-01
• 期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
• 影响因子: 5.2
• 作者: Hirata, Takeshi;Watanabe, Masami;Kumon, Hiromi
• 通讯作者: Kumon, Hiromi

Intraperitoneal administration of an adenovirus vector carrying REIC/Dkk-3 suppresses peritoneal dissemination of scirrhous gastric carcinoma

• DOI: 10.3892/or.2011.1149
• 发表时间: 2011-04-01
• 期刊: ONCOLOGY REPORTS
• 影响因子: 4.2
• 作者: Than, Swe Swe;Kataoka, Ken;Huh, Nam-Ho
• 通讯作者: Huh, Nam-Ho