The role of autophagy in cardioprotection by volatile anesthetics

自噬在挥发性麻醉药心脏保护中的作用

• 批准号: 23593008
• 负责人: MIYAMAE Masami
• 金额: $ 3.24万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23593008/
• 关键词: sevoflurane; heart; cardioprotection; autophagy; ischemia

Sevoflurane preconditioning(SEVO-PC) is lost if the ischemic insult is too long. We examined whether induction of autophagy prolongs SEVO-PC protection during a longer ischemic insult.Isolated guinea pigs hearts were subjected to 30 or 45min ischemia,followed by 120min reperfusion. Anesthetic preconditioning was elicited with 2% sevoflurane for ten minutes prior to ischemia. Infarct size sparing effect of SEVO-PC was seen in 30min ischemia, but not 45min ischemia.Induction of autophagy by chloramphenicol prior to ischemia restored the infarct size limiting effect of SEVO-PC lost by 45min ischemia. Western blot analysis showed that expression of Akt and glycogen synthase kinase 3beta was enhanced by induction of autophagy.The effect of autophagy on calcium-induced mitochondrial permeability transition pore(MPTP) opening in isolated mitochondria from cardiomyocytes was assessed.The restoration of SEVO-PC lost by 45min ischemia is associated with enhanced inhibition of MPTP by autophagy.

如果缺血性侮辱太长，则损失Sevoflurane预处理（SEVO-PC）。我们检查了自噬的诱导是否在较长的缺血性侮辱期间延长了塞沃-PC的保护。分离的豚鼠心脏受到30或45分钟的缺血，然后进行120分钟的再灌注。在缺血前十分钟，用2％的七氟硫烯引起麻醉预处理。在30分钟的缺血中观察到了SEVO-PC的梗死大小释放效应，但在45分钟的缺血中却没有。在缺血之前通过氯霉素诱导自噬，恢复了45min缺血损失的SEVO-PC的梗塞大小的限制效应。蛋白质印迹分析表明，通过诱导自噬来增强AKT和糖原合酶激酶3BetA的表达。自噬对钙诱导的线粒体通透性过渡孔（MPTP）在隔离的线粒体中的开放中的钙软骨增强的损失ISCHINED ISCHINED ISCHONIND ISCHON。由自噬的MPTP。

项目成果

Synergistic effects of necroptosis and apoptosis inhibition against myocardial ischemia- reperfusion injury

坏死性凋亡和细胞凋亡抑制对心肌缺血再灌注损伤的协同作用

• 发表时间: 2013
• 作者: Koshinuma S;Miyamae M;Kaneda K;Figueredo VM;Kotani J
• 通讯作者: Kotani J

心筋虚血再灌流障害におけるnecroptosisの関与

坏死性凋亡参与心肌缺血再灌注损伤

• 发表时间: 2012
• 作者: 山内健介;野上晋ノ介;田中謙光;金氏 毅;宮本郁也;高橋 哲;金高弘恭;越沼静,宮前雅見,金田一弘,大草知佳,稲村吉高,大西杏奈,小谷順一郎
• 通讯作者: 越沼静,宮前雅見,金田一弘,大草知佳,稲村吉高,大西杏奈,小谷順一郎

心筋虚血前のオートファジーの誘導は長時間虚血により消失したセボフルランプレコンディショニング効果を回復させる

心肌缺血前诱导自噬可恢复因长期缺血而消除的七氟烷预处理作用。

• 发表时间: 2012
• 作者: K Hoshi;H Kawaki;I Takahashi;N Takeshita;M Seiryu;S A Murshid;T Masuda;T Anada;R Kato;H Kitaura;O Suzuki;T Takano-Yamamoto;塩見真由美,宮前雅見,金田一弘,稲村吉高,竹村元三,南敏明
• 通讯作者: 塩見真由美,宮前雅見,金田一弘,稲村吉高,竹村元三,南敏明

Periodontal disease is an independent predictor of intracardiac calcification.

• DOI: 10.1155/2013/854340
• 发表时间: 2013
• 期刊: BioMed research international
• 作者: Pressman GS;Qasim A;Verma N;Miyamae M;Arishiro K;Notohara Y;Crudu V;Figueredo VM
• 通讯作者: Figueredo VM

Lidocaine protecs against myocardial ischemia-reperfusion injury in anesthetized rabbits

利多卡因对麻醉兔心肌缺血再灌注损伤的保护作用

• 发表时间: 2013
• 期刊: J Osaka Dent Univ
• 作者: Yamabayashi K;Momota Y;Miyamae M;Kaneda K;Kotani J
• 通讯作者: Kotani J