Study on the expression status of CEA gene family in cancer and surrounding normal tissue and on the interaction between cancer and normal mesenchymal cells.

研究癌及周围正常组织中CEA基因家族的表达状况以及癌与正常间充质细胞之间的相互作用。

• 批准号: 23592956
• 负责人: KOIKE HIROFUMI
• 金额: $ 3.16万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592956/
• 关键词: CEA遺伝子ファミリー; 口腔癌; 遺伝子発現ベクター; CEA遺伝子群; 浸潤; 転移; CEA遺伝子family

Of the CEA family genes, CEACAM1 was the most depressed expression in the oral squamous cell carcinoma (OSCC)-derived cell lines. CEACAM1 expression in clinical samples detected strong signal in normal epithelium and very weak signal in tumor. There is a significant inverse correlation between signal intensity and tumor size/stage. Expression vector including cDNA of CEACAM1 was constructed and two OSCC derived cell lines were transformed by the vector, and transformant indicated CEACAM1 suppresses the growth and invasion of the tumor. Then, co-culture of the tarnsformant and normal fibroblasts was performed, resulting in the increased expression of CEACAM1 in fibroblasts. The decreased expression of the representative genes, CCND1, CDNK1, MYC, of the Wnt signaling pathway was observed in the co-cultured fibroblasts. Suppressing possibility was suggested tumor growth might be suppressed by CEACAM1 expressed from the surrounding normal tissue cells.

在 CEA 家族基因中，CEACAM1 在口腔鳞状细胞癌 (OSCC) 衍生细胞系中的表达最受抑制。临床样本中的CEACAM1表达在正常上皮中检测到强信号，在肿瘤中检测到非常弱的信号。信号强度和肿瘤大小/阶段之间存在显着的负相关。构建了包含CEACAM1 cDNA的表达载体，并用该载体转化两个OSCC来源的细胞系，转化体表明CEACAM1抑制肿瘤的生长和侵袭。然后，将畸形细胞与正常成纤维细胞共培养，导致成纤维细胞中CEACAM1表达增加。在共培养的成纤维细胞中观察到Wnt信号通路的代表性基因CCND1、CDNK1、MYC的表达降低。抑制可能性表明肿瘤生长可能被周围正常组织细胞表达的CEACAM1抑制。