Vasogenic brain edema in the spontaneous hypertensive rat

自发性高血压大鼠血管源性脑水肿

• 批准号: 23592094
• 负责人: HAYASHI KENTARO
• 金额: $ 3.08万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592094/
• 关键词: 脳血管障害; 血液脳関門; 血管原性脳浮腫; blood brain barrier; hypertension; glioma; hyperglicemia; ラット

We used in vitro blood-brain barrier (BBB) models with primarily cultured BBB-related cells from rats. Treatment of AGEs did not affect the transendothelial electrical resistance (TEER) in the BBB model under normoxia, but there was a significant decrease in TEER under 3-h oxygen glucose deprivation (OGD)/24-h reoxygenation conditions with AGEs. Cilostazol inhibited decreases in TEER induced by 3-h OGD/24-h reoxygenation with AGEs. Furthermore, while AGEs increased the production of extracellular transforming growth factor (TGF)-beta1, cilostazol inhibited the production of extracellular TGF- beta 1 and restored the integrity of tight junctions.

我们使用体外血脑屏障 (BBB) 模型，主要培养来自大鼠的 BBB 相关细胞。 AGEs的治疗并不影响常氧下BBB模型的跨内皮电阻(TEER)，但在3小时氧糖剥夺(OGD)/24小时复氧条件下AGEs显着降低TEER。西洛他唑抑制 3 小时 OGD/24 小时 AGE 复氧诱导的 TEER 降低。此外，虽然 AGE 增加了细胞外转化生长因子 (TGF)-β1 的产生，但西洛他唑抑制了细胞外 TGF-β1 的产生并恢复了紧密连接的完整性。