The role of proteoglycans in the modulation of EGFR family and it's ligand in lung fibrosis

蛋白多糖在调节EGFR家族及其配体在肺纤维化中的作用

• 批准号: 23591139
• 负责人: JO Taisuke
• 金额: $ 3.33万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591139/
• 关键词: 肺線維症; プロテオグリカン; EGFRファミリー; マウス; ブレオマイシン; EGFR

Idiopathic Pulmonary Fibrosis is a progressive disease with poor clinical outcome. And yet treatment strategy has not been well-established. In this study we examined the expression of Epidermal Growth Factor Receptors (EGFR) and it`s ligands in isolated lungs from mouse model of bleomycin-induced pulmonary fibrosis. We compared C56BL6 and Balb/c, a strain known to show stronger fibrotic change and mild fibrotic change with bleomycin, respectively. Proteoglycan expression was concomitantly evaluated with intention to explore the association with EGFR and EGFR ligands. Lungs from C56BL6 mice appeared to have apparent fibrotic changes and higher collagen content compared to Balb/c mice. Expression of amphiregulin, epiregulin, versican and decorin seemed to be lower in C56BL6 compared Balb/c mice, whilst EGFR seemed to be highly expressed in C56BL6. These findings provide bases for further investigation.

特发性肺纤维化是一种进行性疾病，临床结果不佳。然而治疗策略尚未制定。在这项研究中，我们检测了博莱霉素诱导肺纤维化小鼠模型离体肺中表皮生长因子受体 (EGFR) 及其配体的表达。我们比较了 C56BL6 和 Balb/c，一种已知在博莱霉素作用下分别表现出更强纤维化变化和轻度纤维化变化的菌株。同时评估蛋白多糖表达，旨在探索与 EGFR 和 EGFR 配体的关联。与 Balb/c 小鼠相比，C56BL6 小鼠的肺似乎有明显的纤维化变化和更高的胶原蛋白含量。与 Balb/c 小鼠相比，C56BL6 中双调蛋白、上皮调节蛋白、多功能蛋白聚糖和核心蛋白聚糖的表达似乎较低，而 EGFR 似乎在 C56BL6 中高度表达。这些发现为进一步调查提供了基础。

项目成果

Interleukin-17A Enhances Interferon- Gamma-Induced CXCL10/IP-10 Production In BEAS-2B Human Bronchial Epithelial Cells

Interleukin-17A 增强 BEAS-2B 人支气管上皮细胞中干扰素 γ 诱导的 CXCL10/IP-10 产生

• 发表时间: 2013
• 作者: Yasuhiro Yamauchi;Taisuke Jo;Masafumi Horie;Yu Mikami;Satoshi Noguchi;Osamu Narumoto;Kazutaka Takami;Tadashi Kohyama;Hajime Takizawa;Takahide Nagase
• 通讯作者: Takahide Nagase

Histamine induces human lung fibroblast-mediated collagen gel contraction via histamine H1 receptor

• DOI: 10.3109/01902148.2014.900155
• 发表时间: 2014-06-01
• 期刊: EXPERIMENTAL LUNG RESEARCH
• 影响因子: 1.7
• 作者: Horie, Masafumi;Saito, Akira;Kohyama, Tadashi
• 通讯作者: Kohyama, Tadashi

Tumor Necrosis Factor Super Family Member LIGHT Induces CXCL8 Production In BEAS-2B Human Bronchial Epithelial Cells Via Erk1/2 Pathway

肿瘤坏死因子超家族成员 LIGHT 通过 Erk1/2 途径诱导 BEAS-2B 人支气管上皮细胞产生 CXCL8

• 发表时间: 2013
• 作者: Yu Mikami;Yasuhiro Yamauchi;Masafumi Horie;Satoshi Noguchi;Osamu Narumoto;Taisuke Jo;Tadashi Kohyama;Hajime Takizawa;Takahide Nagase
• 通讯作者: Takahide Nagase

Interleukin-17A Enhances Interferon-Gamma-Induced CXCL10/IP-10 Production In BEAS-2B Human Bronchial Epithelial Cells.

Interleukin-17A 增强 BEAS-2B 人支气管上皮细胞中干扰素 γ 诱导的 CXCL10/IP-10 产生。

• 发表时间: 2013
• 作者: Yasuhiro Yamauchi;Taisuke Jo;Masafumi Horie;Yu Mikami;Satoshi Noguchi;Osamu Narumoto;Kazutaka Takami;Tadashi Kohyama;Hajime Takizawa;Takahide Nagase,
• 通讯作者: Takahide Nagase,