Detection of novel actions of kampo medicines for lipid metabolism by lipidomic analysis

通过脂质组学分析检测汉方药物对脂质代谢的新作用

• 批准号: 23590873
• 负责人: SHIRO Watanabe
• 金额: $ 3.24万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590873/
• 关键词: 胆汁酸; トリグリセリド; 一価不飽和脂肪酸; ステアリン酸CoA不飽和化酵素1; ステアリン酸CoA不飽和化酵素１; コレステロールエステル; トランスアミナーゼ; コール酸; 肝傷害; エイコサペンタエン酸; リポプロテインリパーゼ; 胆汁酸; トリグリセリド; 一価不飽和脂肪酸; ステアリン酸CoA不飽和化酵素1; ステアリン酸CoA不飽和化酵素１; コレステロールエステル; トランスアミナーゼ; コール酸; 肝傷害; エイコサペンタエン酸; リポプロテインリパーゼ

The administration of Boiogito (BOT) was shown to ameliorate liver injury in the mice fed a cholate (CA)-supplemenetd diet. This effect was associated with a partial restoration of the reduction in liver triglyceride (TG) content. The levels of gene and protein of stearoyl-CoA desaturase-1 (SCD1) were lowered in the mice fed the CA-supplemented diet, which were elevated by BOT administration. These results suggest that the amelioration of CA-induced liver injury by BOT administration may be due to the restoration of reduced synthesis of liver TG through the facilitation of desaturation of saturated fatty acids, which is required in the efficient TG synthesis in liver. Alternatively, the reduction in liver TG levels might be a consequence of the amelioration of CA-induced liver injury by BOT administration. Thus, our lipidomic analysis revealed a novel mechanism involved in the modulation of lipid metabolism by a kampo medicine during bile acid-induced steatohepatitis in the mice.

在喂食challic（CA） - 舒普莱梅内特饮食的小鼠中，boiogito（Bot）的给药被证明可以改善肝损伤。这种影响与部分恢复肝甘油三酸酯（TG）含量的降低有关。在喂养Ca供应的饮食的小鼠中，降低了stearoyl-COA去饱和酶-1（SCD1）的基因和蛋白质水平，这些饮食被Bot给药升高。这些结果表明，通过机器人给药来改善CA诱导的肝损伤可能是由于通过促进饱和饱和脂肪酸的去饱和度恢复了肝脏TG的合成降低，这在有效的TG肝脏中所必需。或者，肝Tg水平的降低可能是由于机器人给药减轻CA诱导的肝损伤的结果。因此，我们的脂肪组分析表明，在小鼠胆汁酸诱导的脂肪性肝炎期间，一种新的机制参与了坎波医学对脂质代谢的调节。