SEARCHING OF SPECIFIC PROTEINS PRODUCED IN GALLBLADDER CANCER TISSUES FOR THE EARLY DETECTION OF THE CANCER

搜索胆囊癌组织中产生的特定蛋白质以早期检测癌症

• 批准号: 23590759
• 负责人: TSUCHIYA Yasuo
• 金额: $ 3.24万
• 依托单位: Niigata University of Health and Welfare
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590759/
• 关键词: 胆嚢がん; スクリーニング; 特異蛋白; 質量分析; 腫瘍マーカー; 特異蛋白質; 国際情報交換; チリ; プロテオミクス解析

Our operating procedure to detect and identify the specific proteins as the biomarker for the early detection of gallbladder cancer was as follows: preparation of paraffin sections from formalin-fixed paraffin-embedded gallbladder cancer tissue blocks, deparaffinization, stain sections on a microscope by Periodic acid-Schiff stain, excision of tissue section by laser microdissection, on-site direct digestion, purification of the samples using the C18 Stage Tip, concentration measurement of the peptide solutions by micro bicinchoninic acid protein assay, LC/MS/MS analysis.A total of 15 samples were collected: 3 from normal cell (group I), 6 from cancer cell (group II), and 6 from advanced cancer cell (group III). The average numbers of detected proteins from each group were 993, 1,291, and 1,109. In these proteins, the group specific proteins were 306, 1,148, and 930. The new biomarkers for the early detection of gallbladder cancer may be found to examine these proteins closely.

Our operating procedure to detect and identify the specific proteins as the biomarker for the early detection of gallbladder cancer was as follows: preparation of paraffin sections from formalin-fixed paraffin-embedded gallbladder cancer tissue blocks, deparaffinization, stain sections on a microscope by Periodic acid-Schiff stain, excision of tissue section by laser microdissection, on-site direct digestion,使用C18阶段尖端，通过微双氨基甲酸蛋白测定法，LC/MS/MS分析对肽溶液的浓度测量进行纯化。总共收集了15个样品：3个正常细胞（I组）（I组），来自癌细胞II（II组）的6个样品（II），6个来自晚期癌细胞（III组）。 每组检测到的蛋白质的平均数量为993、1291和1,109。 在这些蛋白质中，特异性蛋白质为306、1,148和930。可以发现，可以发现胆囊癌的新生物标志物可密切检查这些蛋白质。

项目成果

Proteomic Analysis of Paraffin-Embedded Gallbladder Cancer Tissues

石蜡包埋胆囊癌组织的蛋白质组学分析

• 发表时间: 2013
• 作者: Asai T;Tsuchiya Y;et al
• 通讯作者: et al