Resistance mechanisms of the diamondback moth against various insecticides

小菜蛾对多种杀虫剂的抗性机制

• 批准号: 23580072
• 负责人: SONODA Shoji
• 金额: $ 3.49万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580072/
• 关键词: アセチルコリンエステラーゼ; コナガ; 遺伝子重複; アミノ酸変異; 有機リン剤; 殺虫剤抵抗性; RNA editing; RNA variegation; ピレスロイド剤; ナトリウムチャネル

This study examined the acetylcholinesterase 1 gene (AChE1) in Plutella xylostella strains with different sensitivities to acephate. Multiple haplotypes of the gene were found in the field-collected strains including distinct haplotypes carrying one or both previously reported mutations (A298S and G324A). Moreover, sequencing results indicated the presence of duplicated copies of the gene in the fieldcollected strains. No correlation was found between copy numbers of AChE1 and levels of resistance to acephate suggesting that extensive AChE1 duplication is not a major resistance factor at least in some P. xylostella strains. Proportions of the A298S and G324A mutations showed no correlation with levels of resistance to acephate. This suggests that acephate resistance of P. xylostella is complex and cannot be evaluated based on the AChE1 copy number or proportions of the resistance mutations alone.

这项研究检查了乙酰胆碱酯酶1基因（ACHE1）中的木质酸紫罗兰菌菌株，对静脉敏感性不同。该基因的多种单倍型在现场收集的菌株中发现，包括带有一种或两个先前报道的突变（A298S和G324A）的不同单倍型。此外，测序结果表明在现场收集菌株中存在该基因的重复拷贝。在ACHE1的拷贝数和对静脉的抗性水平之间未发现相关性，这表明至少在某些木质假单胞菌菌株中，广泛的ACHE1重复并不是主要的抗性因子。 A298S和G324A突变的比例与对屈服的抗性水平无关。这表明木质杆菌的屈服性是复杂的，无法基于ACHE1拷贝数或仅靠抗性突变的比例进行评估。

项目成果

コナガのピレスロイド剤抵抗性-野外におけるナトリウムチャネルのアミノ酸変異-

小菜蛾拟除虫菊酯耐药性-田间钠通道氨基酸突变-

• 作者: Sato T;Matsuda K;Ezawa T;Cheng W;Tawaraya K;園田昌司
• 通讯作者: 園田昌司

Sodium channel mutations associated with pyrethroid resistance and their frequencies in the field strains of the diamondback moth, Plutella xylostella collected at different Asian countries

与拟除虫菊酯抗性相关的钠通道突变及其在亚洲不同国家收集的小菜蛾田间品系中的频率

• 发表时间: 2011
• 作者: Sonoda;S
• 通讯作者: S

コナガの合成ピレスロイド剤抵抗性に関わるナトリウムチャネルのアミノ酸変異の頻度-日本、中国、タイ系統の比較

小菜蛾钠通道氨基酸突变频率与合成拟除虫菊酯抗性相关——日本、中国和泰国菌株的比较

• 发表时间: 2012
• 作者: 園田昌司;et al
• 通讯作者: et al

難防除害虫の薬剤抵抗性メカニズム

难治害虫的耐药机制

• 发表时间: 2012
• 作者: 安佛 尚志;二河 成男;田中康次郎;深津 武馬;園田昌司
• 通讯作者: 園田昌司

Organophosphate resistance in the laboratory and field strains of the diamondback moth

小菜蛾实验室和田间菌株的有机磷抗性

• 发表时间: 2012
• 作者: Sonoda;S
• 通讯作者: S