Detection of gastrointestinal cancer biomarkers by next-generation mass spectrometry and comprehensive gene expression analysis

通过新一代质谱法和综合基因表达分析检测胃肠癌生物标志物

• 批准号: 23390320
• 负责人: SHIMADA Yutaka
• 金额: $ 11.98万
• 依托单位: Kyoto University (2013)University of Toyama (2011-2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390320/
• 关键词: バイオマーカー; 食道癌; miRNA; 早期発見; 質量分析; 次世代質量顕微鏡

We here evaluated a next-generation mass spectrometer and found that it not suitable for a comprehensive protein analysis of blood. Therefore, we performed a microRNA analysis of blood. To exclude individual differences between patients, we focused on esophageal mucosal cancer patients who were scheduled to undergo endoscopic mucosal resection (EMR) in the present study. Forty-three patients were recruited from 3 institutes. We analyzed the expression of miRNAs in pre-treatment as well as post-treatment blood samples that were collected from 31 patients. Significant differences were observed in the expression of 11 miRNAs between pre-treatment and post-treatment samples. We will analyze these miRNAs in 30 patients with advanced esophageal cancer in a future study and evaluate their efficacy as biomarkers of esophageal cancer.

我们在这里评估了下一代质谱仪，发现它不适合对血液进行全面的蛋白质分析。因此，我们对血液进行了 microRNA 分析。为了排除患者之间的个体差异，我们在本研究中重点关注计划接受内镜粘膜切除术（EMR）的食管粘膜癌患者。从 3 个研究所招募了 43 名患者。我们分析了从 31 名患者收集的治疗前和治疗后血液样本中 miRNA 的表达。处理前和处理后样本中 11 种 miRNA 的表达存在显着差异。我们将在未来的研究中分析 30 名晚期食管癌患者的这些 miRNA，并评估它们作为食管癌生物标志物的功效。

项目成果

MicroRNA profiling of gastric cancer patients from formalin-fixed parafifin-embedded samples

福尔马林固定石蜡包埋样本中胃癌患者的 MicroRNA 分析

• 发表时间: 2011
• 期刊: Oncology letters
• 影响因子: 2.9
• 作者: Osawa S;Shimada Y;Sekine S;Okumura T;Nagata T;Fukuoka J;Tsukada K
• 通讯作者: Tsukada K

Expression Analysis of iPS Cell Inductive Genes in Esophageal Squamous Cell Carcinoma by Tissue Microarray

组织微阵列技术分析食管鳞癌iPS细胞诱导基因的表达

• 发表时间: 2012
• 期刊: Anticancer Res
• 影响因子: 2
• 作者: Shimada Y;Okumura T;Sekine S;Moriyama M;Sawada S;Matsui K;Y oshioka I;Hojo S;Y oshida T;Nagata T;Fukuoka J;Tsukada K
• 通讯作者: Tsukada K

Critical Role for the Receptor Tyrosine Kinase EPHB4 in Esophageal Cancers

• DOI: 10.1158/0008-5472.can-12-0915
• 发表时间: 2013-01-01
• 期刊: CANCER RESEARCH
• 影响因子: 11.2
• 作者: Hasina, Rifat;Mollberg, Nathan;Salgia, Ravi
• 通讯作者: Salgia, Ravi

Barrett’s adenocarcinoma arising 30 years after a Nissen fundoplication

Nissen 胃底折叠术 30 年后出现 Barrett 腺癌

• DOI: 10.1007/s10388-013-0370-z
• 发表时间: 2013
• 期刊: Esophagus
• 影响因子: 2.4
• 作者: Shimasaki T;Kawakami K;Minamoto T;et al;西尾禎治;Shimada Y
• 通讯作者: Shimada Y

MicroRNA-141 confers resistance to cisplatin-induced apoptosis by targeting YAP1 in human esophageal squamous cell carcinoma

• DOI: 10.1038/jhg.2011.1
• 发表时间: 2011-04-01
• 期刊: JOURNAL OF HUMAN GENETICS
• 影响因子: 3.5
• 作者: Imanaka, Yukako;Tsuchiya, Soken;Tsujimoto, Gozoh
• 通讯作者: Tsujimoto, Gozoh