Elucidation of the suppression mechanism to activation of cancer stem cells by hyperthermia

阐明热疗抑制癌症干细胞活化的机制

• 批准号: 23390303
• 负责人: TAKAHASHI AKIHISA
• 金额: $ 11.65万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390303/
• 关键词: 放射線治療; 温熱治療; 感受性; がん幹細胞; DNA二本鎖切断; 放射線治療学

This study examined to clarify the heat sensitivity in X-ray resistant cells from human tongue cancer cells. Our results show that heat sensitivity was not significant difference between X-ray resistant cells and parental cells. The data of flow cytometory showed that cancer stem-like cells were significantly decreased after heating as compared with exposure to X-rays at iso-survival doses in both cells.In addition, we examined the effect of the inhibitor of HR repair or NHEJ repair for DSBs on heat sensitivity. Heat sensitivity was not affected by NHEJ inhibitor. On the other hand, HR inhibitor was able to enhance heat sensitivity. These results suggest that the heat sensitivity may be enhanced by the suppression of HR repair. In addition, these findings provide support for the concept that heat may lead to the induction of DSBs.

这项研究旨在阐明人类舌癌细胞的 X 射线抗性细胞的热敏感性。我们的结果表明，X射线抵抗细胞和亲本细胞之间的热敏感性没有显着差异。 流式细胞术数据显示，与暴露于等存活剂量的X射线相比，加热后两种细胞中的癌症干细胞样细胞均显着减少。此外，我们检查了HR修复或NHEJ修复抑制剂的效果DSB 的热敏感性。 NHEJ 抑制剂不影响热敏感性。另一方面，HR抑制剂能够增强热敏感性。这些结果表明热敏感性可能通过抑制 HR 修复而增强。此外，这些发现支持了热可能导致 DSB 诱导的概念。

项目成果

X線抵抗性細胞の重粒子線感受性について

关于X射线抵抗细胞对重离子辐射的敏感性

• 发表时间: 2011
• 作者: 高橋昭久;吉田由香里;馬洪玉;桑原義和;福本学;金井達明;中野隆史
• 通讯作者: 中野隆史

Hyperthermic sensitization efficacy as a target of homologous recombination repair pathways in the oral cancer cells.

热敏化功效作为口腔癌细胞同源重组修复途径的目标。

• 发表时间: 2012
• 作者: Atsuhisa Kajihara;Yosuke Nakagawa;Eiichiro Mori;Akihisa Takahashi;Tadaaki Kirita;Takeo Ohnishi
• 通讯作者: Takeo Ohnishi

Heat treatment as well as X-ray-irradiation depresses gastric mucosal damage induced with restraint plus water-immersion stress through HSP-induction in mice.

热处理和 X 射线照射可抑制小鼠 HSP 诱导的约束加水浸应激引起的胃粘膜损伤。

• 发表时间: 2012
• 作者: Akihisa Takahashi;Xiaoming Su;Rikio Yamagata;Takeo Ohnishi
• 通讯作者: Takeo Ohnishi

Cell lethality by carbon- Ion beams in X-ray resistant cells.

碳离子束对 X 射线抵抗细胞的细胞致死率。

• 发表时间: 2012
• 作者: Akihisa Takahashi;Yukari Yoshida;Hong Ma;Yoshikazu Kuwabara;Manabu Fukumoto;Tatsuaki Kanani;Takashi Nakano
• 通讯作者: Takashi Nakano

Achievement of the first life science experiments in the Japanese Experimental Module "Kibo" of the ISS

在国际空间站的日本实验舱“Kibo”中完成首次生命科学实验

• 发表时间: 2011
• 期刊: Int. Sym. Space Tec. Sci
• 作者: Takahashi;A. & T. Ohnishi
• 通讯作者: A. & T. Ohnishi