Determination of optimum conditions for growing symbiotic dinoflagellates, Amphidinium species, and development of methods for its rapid mass culture

共生甲藻、两栖藻类生长最佳条件的确定及其快速大规模培养方法的开发

• 批准号: 23380151
• 负责人: KITAYA Yoshiaki
• 金额: $ 12.23万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23380151/
• 关键词: 生物環境調節; 生物生産システム; 渦鞭毛藻; 培養; 藻類培養; 藻類培養システム; 微細藻; CO2; O2; 光合成; 比増殖速度

Symbiotic dinoflagellates, Amphidinium species, are expected to be pharmaceutically useful microalgae, because they produce antitumor macrolides. A microalgae production system with a large number of cells at a high density has been developed to produce macrolide compounds efficiently. In this study, effects of culture conditions on the cellular multiplication of dinoflagellates were investigated to determine the optimum culture conditions for obtaining high yield of the microalgae. The maximum specific growth rate was obtained at a temperature of 26oC, PPFDs of0.05-0.07 mmol m-2 s-1, photoperiod of 12 h d-1, O2 concentration of 5% and CO2 concentration of 0.1%. The results demonstrate that Amphidinium species could efficiently multiply under the condition with relatively low light intensities and low O2 concentrations with additional inorganic nutrients.The optimal depth of cuilture solution was 10 mm.

共生甲藻（Amphidinium 物种）有望成为药学上有用的微藻，因为它们产生抗肿瘤大环内酯。已经开发出一种具有大量高密度细胞的微藻生产系统，可以有效地生产大环内酯化合物。在本研究中，研究了培养条件对甲藻细胞增殖的影响，以确定获得高产微藻的最佳培养条件。在温度26℃、PPFD 0.05-0.07 mmol m-2 s-1、光周期12 h d-1、O2浓度5%、CO2浓度0.1%时获得最大比生长率。结果表明，在相对较低的光照强度和较低的O2浓度以及额外的无机营养物质的条件下，水生藻类物种能够高效繁殖。培养液的最佳深度为10 mm。

项目成果

Effects of O_2 and CO_2 concentrations on cellular multiplication and net photosynthetic rates of dinoflagellates, Amphidinium species

O_2和CO_2浓度对甲藻、两栖类细胞增殖和净光合速​​率的影响

• 发表时间: 2012
• 作者: Kitaya;Y.;Xiao;L
• 通讯作者: L