Construction of the technical bases for conservation genetics in Aegagropila linnaei in Lake Akan

阿寒湖林内山羊草保护遗传学技术基地建设

• 批准号: 22710239
• 负责人: NISHIZAWA Toru
• 金额: $ 2.5万
• 依托单位: University of Tsukuba (2011-2012)National Institute for Environmental Studies (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22710239/
• 关键词: マリモ; 阿寒湖; 分子マーカー; マイクロサテライト; 遺伝的多様性; 個体群保全; 個体群復元

I developed microsatellite markers in Aegagropia linnaei, a green algae that distributes in Lake Akan. However, polymorphic loci were not captured in this study duration. I also considered optimum conditions which allow the nuclear-phase analysis that covers a single fragment specimen. Due to extremely low DNA content in a single fragment specimen, the nuclear-phase analysis using flow cytometry was impossible.

我在 Aegagropia linnaei（一种分布在阿寒湖的绿藻）中开发了微卫星标记。然而，在本研究期间并未捕获多态性基因座。我还考虑了允许覆盖单个碎片样本的核相分析的最佳条件。由于单个片段样本中的 DNA 含量极低，因此无法使用流式细胞术进行核相分析。