Dual regulation of secretion system of periodontal virulence factors by novel two-component system and ECF sigma factor

新型双组分系统和ECF西格玛因子对牙周毒力因子分泌系统的双重调节

• 批准号: 22659332
• 负责人: NAKAYAMA Koji
• 金额: $ 2.01万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659332/
• 关键词: 遺伝子; 発現調節; 二成分制御系; シグマ因子; 歯周病; 歯学; 細菌; プロテアーゼ; 膜輸送

We investigated regulatory mechanism of secretion system of pathogenic proteases/ adhesins of the periodontopathogen Porphyromonas gingivalis. Secretion of P. gingivalis gingipain proteases/ adhesins depends on a protein secretion system, Por secretion system(PorSS). We isolated 12 gingipain secretion-deficient mutants and found that two of them were deficient in a two-component system, which is a prokaryotic signaling system and consists of the sensor histidine kinase PorY and response regulator PorX. In this study, we constructed recombinant PorY and PorX proteins and found that PorY was autophosphorylated and the phosphoryl residue of PorY was transferred to PorX. PorY could bind to PorX as revealed by Surface plasmon resonance(BIACORE) analysis. Gene expression of component proteins of the PorSS was also regulated by one of ECF sigma factors, PGN0274, but the PorY-PorX system and the PGN0274 sigma factor independently regulate gene expression of component proteins of the PorSS, suggesting that the PorSS is regulated by multiple regulatory mechanisms.

我们研究了牙周病原菌牙龈卟啉单胞菌致病性蛋白酶/粘附素分泌系统的调节机制。牙龈卟啉单胞菌牙龈蛋白酶/粘附素的分泌依赖于蛋白质分泌系统，Por分泌系统(PorSS)。我们分离了 12 个牙龈蛋白酶分泌缺陷突变体，发现其中两个缺乏双组分系统，该系统是原核信号系统，由传感器组氨酸激酶 PorY 和反应调节器 PorX 组成。在本研究中，我们构建了重组PorY和PorX蛋白，发现PorY被自磷酸化，并且PorY的磷酰基残基被转移到PorX上。表面等离子共振 (BIACORE) 分析表明，PorY 可以与 PorX 结合。 PorSS 组成蛋白的基因表达也受到 ECF sigma 因子之一 PGN0274 的调节，但 PorY-PorX 系统和 PGN0274 sigma 因子独立调节 PorSS 组成蛋白的基因表达，表明 PorSS 受到多种因素的调节。监管机制。

项目成果

Tetratricopeptide Repeat Protein-Associated Proteins Contribute to the Virulence of Porphyromonas gingivalis

• DOI: 10.1128/iai.01448-09
• 发表时间: 2010-06-01
• 期刊: INFECTION AND IMMUNITY
• 影响因子: 3.1
• 作者: Kondo, Yoshio;Ohara, Naoya;Nakayama, Koji
• 通讯作者: Nakayama, Koji

比較ゲノム解析から発見されたグラム陰性細菌の新規タンパク分泌機構

通过比较基因组分析发现革兰氏阴性菌的新蛋白质分泌机制

• 发表时间: 2010
• 作者: Kaneda M;Zhang D;Bhattacharjee R;Nakahama KI;Arii S;Morita I.;中山浩次
• 通讯作者: 中山浩次

Porphyromonas gingivalis cell-induced hemagglutination and platelet aggregation.

• DOI: 10.1111/j.1600-0757.2010.00351.x
• 发表时间: 2010-10
• 期刊: Periodontology 2000
• 影响因子: 18.6
• 作者: K. Nakayama

Porphyromonas gingivalis mutant defective in a putative glycosyltransferase exhibits defective biosynthesis of polysaccharide portions of lipopolysaccharide, decreased gingioain activities, strong autoaggregation and increased biofilm formation.

假定的糖基转移酶缺陷的牙龈卟啉单胞菌突变体表现出脂多糖的多糖部分的生物合成缺陷、牙龈酶活性降低、自聚集强和生物膜形成增加。

• 发表时间: 2010
• 期刊: Infection and Immunity
• 影响因子: 3.1
• 作者: Yamaguchi M;Sato K;Yukitake H;Noiri Y;Ebisu S;Nakayama K
• 通讯作者: Nakayama K

Porphyromonas gingivalis Peptidoglycans Induce Excessive Activation of the Innate Immune System in Silkworm Larvae

• DOI: 10.1074/jbc.m110.112987
• 发表时间: 2010-10-22
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Ishii, Kenichi;Hamamoto, Hiroshi;Sekimizu, Kazuhisa
• 通讯作者: Sekimizu, Kazuhisa