Development of analytical methods of carbon and nitrogen stable isotope ratio in nucleic acids

核酸碳氮稳定同位素比值分析方法的建立

• 批准号: 22658107
• 负责人: NUNOURA Takuro
• 金额: $ 2.34万
• 依托单位: Japan Agency for Marine-Earth Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22658107/
• 关键词: 生物環境; 安定同位体; 微生物; 核酸

The objective of this project was to organize a novel stable isotope technology for nucleotide especially rRNA in order to develop a novel methodology in environmental omics. In this study, we optimized the online separation and detection for deoxyribonucleotide by using high performance liquid chromatography combined with electrospray ionization mass spectrometry(unpublished). However, we could not construct a new methods for purification of nucleotides to use stable isotopic analysis due to several technical difficulties.

该项目的目标是组织一种新型的核苷酸稳定同位素技术，特别是 rRNA，以开发环境组学的新方法。在本研究中，我们利用高效液相色谱结合电喷雾电离质谱（未发表）优化了脱氧核糖核苷酸的在线分离和检测。然而，由于一些技术困难，我们无法构建新的核苷酸纯化方法以使用稳定同位素分析。