Identification of Al-binding peptide

Al结合肽的鉴定

• 批准号: 22658022
• 负责人: MA Jian feng
• 金额: $ 2.34万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22658022/
• 关键词: イネ; アルミニウム; ペプチド; 結合; 結合ペプチド; 耐性; 転写因子

We functionally characterized an ART-regulated gene, OsCDT3. OsCDT3 encodes a predicted peptide of 53 amino acid residues, of which 14(26. 4%) are cysteine residues. OsCDT3 was mainly expressed in the roots and its expression was specifically induced by Al exposure. OsCDT3 was localized to the plasma membrane. Knockdown of OsCDT3 resulted in increased sensitivity to Al in rice. Yeast expressing OsCDT3 showed increased tolerance to Al and decreased accumulation of Al. Our results indicate that OsCDT3 may play a role in detoxifying Al at the apoplast.

我们对 ART 调节基因 OsCDT3 进行了功能表征。 OsCDT3 编码一个由 53 个氨基酸残基组成的预测肽，其中 14 个（26. 4%）是半胱氨酸残基。 OsCDT3主要在根中表达，其表达是由Al暴露特异性诱导的。 OsCDT3 定位于质膜。 OsCDT3 的敲低导致水稻对铝的敏感性增加。表达 OsCDT3 的酵母显示出对 Al 的耐受性增加并减少 Al 的积累。我们的结果表明 OsCDT3 可能在质外体中的铝解毒中发挥作用。

项目成果

A gene encoding a cysteine-rich peptide is involved in rice Al tolerance

编码富含半胱氨酸肽的基因参与水稻铝耐受性

• 发表时间: 2011
• 作者: Xia;J.X.;Yamaji;N.;Ma;J.F.
• 通讯作者: J.F.

A gene encoding a cysteine-rich peptide is involved in rice Al toleranceA gene encoding a cysteine-rich peptide is involved in rice Al tolerance

编码富含半胱氨酸肽的基因参与水稻铝耐受性编码富含半胱氨酸肽的基因参与水稻铝耐受性

• 发表时间: 2011
• 作者: Xia;J. X.;Yamaji;N.;Ma;J. F
• 通讯作者: J. F