Analysis of the relationship between Chemotaxis system and rotational motion of motors of the bacteria by the multi-motor measurements

通过多电机测量分析细菌趋化系统与电机旋转运动的关系

• 批准号: 22657038
• 负责人: ISHIJIMA Akihiko
• 金额: $ 2.23万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22657038/
• 关键词: 1分計測; 生物物理; ナノバイオ; ナノマシン; 分子モーター; バクテリア; 回転計測; モータータンパク; 温度; トルク

The bacteria flagellar motor is a motor of which diameter is only 40nm, and rotate about 1000 rotations per second using flow of ions. E. Coli has about 5 filaments and changes these directions by the information from chemo-receptors. Although, the bacteria has works with highly sophisticated signal transduction, energy conversion, and cooperation system, we have a few information about them. In this research, by fusing nano-measurements system and imaging system, the relationship between each motors, the response of the motors by the chemo-signals, the response of the motor by the external stimuli, the correlation between the local stimulation by the caged-compounds and the response of the cell and the correlation between the regulation of the gene expression an rotational motion of the motor.Using these techniques, we found that there were correlations of the switching of each motors, and this timing has some delay. This delay was depended on the position of the each motors, that suggested that the information from the cell pole propagated as wave. Using caged-compounds, the rotational directions of the motors, CW-bias were changed.

细菌鞭毛电动机是直径仅为40nm的电动机，使用离子流量每秒旋转约1000个旋转。大肠杆菌具有大约5个细丝，并通过化学疗法的信息改变了这些方向。尽管细菌可以与高度复杂的信号转导，能量转换和合作系统一起使用，但我们有一些有关它们的信息。在这项研究中，通过融合纳米测量系统和成像系统，每个电动机之间的关系，化学信号的电动机响应，外部刺激对电动机的响应，笼子刺激的局部刺激之间的相关性 - 组合和细胞的响应以及基因表达的调控之间的相关性。使用这些技术，我们发现每个电动机的切换存在相关性，并且该时机有一定的延迟。该延迟取决于每个电动机的位置，这表明来自细胞极的信息作为波传播。使用笼子化合物，电动机的旋转方向，更改了CW偏置。

项目成果

Role of the polar localization of CheZ for the propagation of intracellular signal in bacterial chemotaxis system

CheZ 极性定位对细菌趋化系统细胞内信号传播的作用

• 发表时间: 2012
• 作者: Fukuoka;H.
• 通讯作者: H.

Construction of the optical system detecting the orientation of molecule from the polarization of fluorescence

根据荧光偏振检测分子方向的光学系统的构建

• 发表时间: 2010
• 作者: Yuichi Inoue;Hajime Fukuoka;Akihiko Ishijima;今野卓;Takahiro Shimizu
• 通讯作者: Takahiro Shimizu

Coordinated Reversal of Flagellar Motors on a Single Escherichia coli Cell

• DOI: 10.1016/j.bpj.2011.03.030
• 发表时间: 2011-05-04
• 期刊: BIOPHYSICAL JOURNAL
• 影响因子: 3.4
• 作者: Terasawa, Shun;Fukuoka, Hajime;Ishijima, Akihiko
• 通讯作者: Ishijima, Akihiko

Two methods of temperature control for single-molecule measurements

• DOI: 10.1007/s00249-010-0667-y
• 发表时间: 2011-05-01
• 期刊: EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
• 影响因子: 2
• 作者: Baker, Matthew A. B.;Inoue, Yuichi;Berry, Richard M.
• 通讯作者: Berry, Richard M.

Adhesion effects of a guanidinium ion appended dendritic "molecular glue" on the ATP-driven sliding motion of actomyosin.

• DOI: 10.1002/anie.200906139
• 发表时间: 2010-04
• 期刊: Angewandte Chemie
• 作者: Kou Okuro;K. Kinbara;Kuniaki Takeda;Y. Inoue;A. Ishijima;T. Aida