Establish a novel assay system to demonstrate the bioavailability of cancer preventive isothiocyanates in cruciferous vegetables by measuring its urinary metabolite

建立一种新的检测系统，通过测量尿代谢物来证明十字花科蔬菜中预防癌症的异硫氰酸盐的生物利用度

• 批准号: 22650174
• 负责人: NAKAMURA Yasushi
• 金额: $ 1.92万
• 依托单位: Kyoto Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22650174/
• 关键词: 生理活性; 癌; 生体機能利用; 細菌; 食品; イソチオシアン酸; 尿; アブラナ科野菜; 摂取基準値; ダイコン; アブラナ科

Our goal is to establish a novel assay system to demonstrate the bioavailability of isothiocyanate by measuring its urinary metabolite, dithiocarbamate. However conventional methods cannot discriminate between dithiocarbamates derived from other cruciferous vegetables vs. test vegetable, thus requiring subjects to fast from cruciferous vegetables during the test. To avoid the inconvenience of fasting from cruciferous vegetables, we developed a novel HPLC method that can fractionate dithiocarbamates in test vegetable from other dithiocarbamates. To validate the assay, we determined levels of urinary dithiocarbamate from human subjects fed with test vegetable-derived isothiocyanate. The time needed for assessment is several days, and does not require the inconvenience of the fasting.

我们的目标是建立一种新型检测系统，通过测量尿代谢物二硫代氨基甲酸盐来证明异硫氰酸盐的生物利用度。然而，传统方法无法区分源自其他十字花科蔬菜和测试蔬菜的二硫代氨基甲酸盐，因此要求受试者在测试期间禁食十字花科蔬菜。为了避免禁食十字花科蔬菜带来的不便，我们开发了一种新型 HPLC 方法，可以将测试蔬菜中的二硫代氨基甲酸盐与其他二硫代氨基甲酸盐分开。为了验证该测定，我们测定了喂食测试植物源异硫氰酸盐的人类受试者的尿二硫代氨基甲酸盐水平。评估所需的时间是几天，并且不需要禁食的不便。