X-ray crystallographic analysis of an ABC transporter

ABC 转运蛋白的 X 射线晶体学分析

• 批准号: 22790512
• 负责人: KODAN Atsushi
• 金额: $ 2.58万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790512/
• 关键词: 生体分子; 蛋白質; 薬学; 癌; ABC蛋白質; X線結晶構造解析; 創薬; 生体分子; 蛋白質; 薬学; 癌; ABC蛋白質; X線結晶構造解析; 創薬

Understanding the mechanism of action of ABC proteins and developing new therapeutic strategies that target ABC proteins would be considerably accelerated with the three-dimensional structures. Crystal structure of an exporter-type ABC transporter from a unicellular eukaryote was determined by the multiwavelength anomalous dispersion(MAD) method using the X-ray diffraction data measured at SPring-8. The resolution obtained in this study is best among those of eukaryotic ABC proteins ever reported to date.

了解ABC蛋白质的作用机理和开发新的治疗策略，这些靶向ABC蛋白将通过三维结构大大加速。来自单细胞真核生物的出口型ABC转运蛋白的晶体结构由使用弹簧-8测得的X射线衍射数据的多波强异常分散（MAD）方法确定。在这项研究中获得的分辨率最好是迄今为止报道的真核ABC蛋白。