Molecular analysis of specification along anteiroposterior axis in body of vertebrates

脊椎动物体内前后轴规格的分子分析

• 批准号: 22770208
• 负责人: KAWAMURA Akinori
• 金额: $ 2.75万
• 依托单位: Saitama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22770208/
• 关键词: 初期発生; 突然変異体; 転写制御機構; ゼブラフィッシュ; エンハンサー; 脊椎動物; 体節形成; 前後軸形成; 発生; 軸形成; パターニング; スクリーング; スクリーニング

During embryogenesis of vertebrates, the repeated and segmental structure, somites, are formed, and subsequently specification along the anteroposterior axis is established in vertebrates, Hox genes have been known to play roles in the specification of identity along the anteroposterior axis. However, the molecular mechanisms underlying this ordered expression patterns of Hox genes remains poorly understood. In this study, we analyzed the zebrafish mutant and identified candidate genes by genetic analyses. In addition, we analyzed the transcriptional regulation of hairy-related gene, which play important roles in the somite patterning. The DNA region downstream of hairy-related gene shows enhancer activity, consistent with the endogenous gene expression. At present, we further proceed toanalyze this DNA region.

在脊椎动物的胚胎发生过程中，形成了重复的节段结构体节，随后在脊椎动物中建立了沿前后轴的规范，已知Hox基因在沿前后轴的身份规范中发挥作用。然而，Hox 基因这种有序表达模式背后的分子机制仍然知之甚少。在这项研究中，我们分析了斑马鱼突变体并通过遗传分析鉴定了候选基因。此外，我们还分析了毛相关基因的转录调控，该基因在体节模式中发挥重要作用。毛相关基因下游的DNA区域显示出增强子活性，与内源基因表达一致。目前，我们进一步对该DNA区域进行分析。

项目成果

生体制御学コース発生生物学研究室HP:

生物防治课程发育生物学实验室HP：