Mechanism of catalytic nitric oxide reduction by membrane-bound nitric oxide reductase : comparison with oxygen reduction reaction

膜结合一氧化氮还原酶催化一氧化氮还原的机制：与氧还原反应的比较

• 批准号: 22770136
• 负责人: TOSHA Takehiko
• 金额: $ 2.75万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22770136/
• 关键词: 一酸化窒素; 金属酵素; 結晶構造解析; 生体分子分光; 膜タンパク質; プロトンポンプ; ヘム; 呼吸酵素; 一酸化窒素還元酵素; 呼吸鎖末端酸化酵素; X線結晶構造解析; 共鳴ラマン分光; 酵素反応機構; 膜蛋白質; 反応中間体

Structures of reduced and CN-bound oxidized nitric oxide reductase(NOR), which is involved in anaerobic respiration chain, were determined by X-ray crystallography. Resonance Raman measurement indicated that the substrate, NO, bound to the active site heme in a bent conformation. The proton transfer mechanism was also examined by molecular dynamics simulation, suggesting the structural factor(s) controlling the catalytic proton transfer in NOR. These findings help us understanding the molecular mechanism of the NO reduction reaction in NOR and the functional conversion from NOR to aerobic respiration enzyme during the molecular evolution.

通过X射线晶体学确定了与厌氧呼吸链有关的还原和CN结合的氧化一氧化氮还原酶（NOR）的结构。共振拉曼测量表明，底物以弯曲构象与活性位点血红素结合。还通过分子动力学模拟检查了质子转移机制，这表明控制了Nor中的催化质子转移的结构因子。这些发现有助于我们理解在分子进化过程中NOR中NO还原反应的分子机制以及从NOR到有氧呼吸酶的功能转化。

项目成果

Recovering Iron from Caged Ferric Oxide Nanominerals in Ferritin

从铁蛋白中的笼状氧化铁纳米矿物质中回收铁

• 期刊: Indian J.Chem.Sect.A
• 作者: T.Tosha;et al.;S.Haldar
• 通讯作者: S.Haldar

Quinol and Ca Binding Sites in Bacterial Nitric Oxide Reductase

细菌一氧化氮还原酶中的喹啉和 Ca 结合位点

• 发表时间: 2010
• 作者: T. Tosha;Y. Matsumoto;S. Nagano;and Y. Shiro;當舎武彦;T.Tosha
• 通讯作者: T.Tosha

呼吸酵素における分子進化の構造基盤

呼吸酶分子进化的结构基础

• 发表时间: 2010
• 作者: T. Tosha;Y. Matsumoto;S. Nagano;and Y. Shiro;當舎武彦;T.Tosha;當舎武彦
• 通讯作者: 當舎武彦

Structural basis for nitrous oxide generation by bacterial nitric oxide reductases

• DOI: 10.1098/rstb.2011.0310
• 发表时间: 2012-05-05
• 期刊: PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
• 影响因子: 6.3
• 作者: Shiro, Yoshitsugu;Sugimoto, Hiroshi;Hino, Tomoya
• 通讯作者: Hino, Tomoya

Crystal structure of quinol-dependent nitric oxide reductase from Geobacillus stearothermophilus

• DOI: 10.1038/nsmb.2213
• 发表时间: 2012-02-01
• 期刊: NATURE STRUCTURAL & MOLECULAR BIOLOGY
• 影响因子: 16.8
• 作者: Matsumoto, Yushi;Tosha, Takehiko;Shiro, Yoshitsugu
• 通讯作者: Shiro, Yoshitsugu