Analysis of functional role of intereukin-6 secreted by tumor-associated macrophage in ovarian cancer ascites

肿瘤相关巨噬细胞分泌的白细胞介素6在卵巢癌腹水中的功能作用分析

• 批准号: 22791529
• 负责人: ISOBE Aki
• 金额: $ 2.25万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791529/
• 关键词: 卵巣癌; 腹膜播種; TAM; IL-6; tocilizumab

Ascites were collected aseptically, and CD11b positive cells(TAMs) were purified by positive selection using magnetic-activate cell sorting(MACS) technology. Co-culture of ovarian cancer cell line, SKOV3ip1 cells, with TAMs significantly enhanced the invasive activity of cancer cells in Matrigel Boyden chamber assay. This enhancement was partially blolcked by the treatment of anti-interleukin(IL)-6 receptor antibody(Tocilizumab). Since SKOV3ip1 cells did not express IL-6 in mRNA and protein level, IL-6 secreted by TAMs were considered to play a key role in ovarian cancer invasion. In vivo ovarian cancer xenografts, i. p. treatment of Tocilizumab significantly reduced peritoneal dissemination and ascites formation. Those results suggested targeting IL-6 produced by TAMs could be a promising therapy for a subset of ovarian cancer patients.

无菌收集腹水，并使用磁性细胞分选（MAC）技术通过阳性选择纯化CD11b阳性细胞（TAM）。卵巢癌细胞系SKOV3IP1细胞的共同培养，TAM显着增强了Matrigel Boyden腔室分析中癌细胞的侵入性活性。通过治疗抗Interleukin（IL）-6受体抗体（Tocilizumab）的治疗，这种增强部分被破坏了。由于SKOV3IP1细胞在mRNA和蛋白质水平上未表达IL-6，因此被认为由TAM分泌的IL-6在卵巢癌入侵中起关键作用。体内卵巢癌异种移植物，i。 p。托珠单抗的治疗显着减少了腹膜传播和腹水形成。这些结果表明，针对TAM产生的IL-6可能是卵巢癌患者的一部分的有前途的疗法。

项目成果

卵巣がん進展時の腹水中に大量に存在する骨髄由来細胞が果たす役割の解明

阐明腹水中大量存在的骨髓来源细胞在卵巢癌进展过程中所起的作用

• 发表时间: 2011
• 作者: Isobe A;Takeda T;Wakabayashi A;Tsuiji K;Li B.;et al.;河邉真也,矢澤隆志,菅野真史,宇佐美陽子,水谷哲也,今道力敬,具云峰,松村健大,宮本薫;磯部晶.澤田健二郎.大八木知史.若林敦子.馬淵誠士.森重健一郎.木村正
• 通讯作者: 磯部晶.澤田健二郎.大八木知史.若林敦子.馬淵誠士.森重健一郎.木村正

Analysis of functional role of interleukin-6 secreted by tumor-associated macrophages in ovarian cancer ascites

肿瘤相关巨噬细胞分泌的白细胞介素6在卵巢癌腹水中的功能作用分析

• 发表时间: 2011
• 作者: Isobe;A.
• 通讯作者: A.

Analysis of functional role of interleukin-6 secreted by tumor-associated macrophage in ovarian cancer ascites.

肿瘤相关巨噬细胞分泌的白细胞介素6在卵巢癌腹水中的功能作用分析。

• 发表时间: 2010
• 作者: Isobe A;Sawada K;Ohyagi C;Mabuchi S;Kimura T.
• 通讯作者: Kimura T.