Mechanismofboneresorption inmiddleearcholesteatoma inthe light of H^+ concentration and H^+-sensing Proteins

H^浓度和H^敏感蛋白对中耳胆脂瘤骨吸收机制的影响

基本信息

批准号：
22591893
负责人：
SUZUKI Hideaki
金额：
$ 2.25万
依托单位：
University of Occupational and Environmental Health, Japan
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2012
项目状态：
已结题

项目摘要

Objective:The etiopathology of bone resorption in cholesteatoma is unclear. We studied pH inmiddle ear cholesteatoma tissue and the permeability of the cholesteatoma epithelium in an attempt to elucidate the mechanism of bone resorption in this disease.Methods:Cholesteatoma tissue was collected from patients with primary acquired middle ear cholesteatoma. The pH of the keratin debris of cholesteatoma was measured using a pH meter. The cholesteatoma epithelium was examined under a confocal laser scanning microscope, and under a transmission electron microscope. Expression of filaggrin in the cholesteatoma tissue was explored by fluorescence immunohistochemistry and by quantitative reverse transcription-polymerase chain reaction.Results: The pH of the keratin debris of cholesteatoma was acidic. The pH of the basal layer of the cholesteatoma epithelium was significantly lower than that of the antrum mucosa. Transmission electron microscope showed distinct penetration of lanthanum in the intercellular space of the basal, spinous and granular layers of the cholesteatoma epithelium, but only a small amount of lanthanum in the granular layer in the normal skin. The expression of filaggrin nlRNA was significantly lower in the cholesteatoma tissue than in the normal skin.Conclusions: These results indicate that acid leakage through the cholesteatonla epitheliunl probably participates in the resorption of the underlying bone structure. The increased permeability of the cholesteatoma epitheliunl may be explained by a decrease in filaggrin expression.

目的：胆脂瘤骨吸收的病因尚不清楚。我们研究了中耳胆脂瘤组织的pH值和胆脂瘤上皮的通透性，试图阐明该病骨吸收的机制。方法：收集原发性中耳胆脂瘤患者的胆脂瘤组织。使用 pH 计测量胆脂瘤角蛋白碎片的 pH 值。在共焦激光扫描显微镜和透射电子显微镜下检查胆脂瘤上皮。采用荧光免疫组化和定量逆转录聚合酶链反应检测胆脂瘤组织中丝聚蛋白的表达。结果：胆脂瘤角蛋白碎片的pH呈酸性。胆脂瘤上皮基底层的pH值明显低于胃窦粘膜的pH值。透射电镜显示胆脂瘤上皮的基底层、棘层和颗粒层的细胞间隙中有明显的镧渗透，而正常皮肤的颗粒层中仅有少量的镧。胆脂瘤组织中丝聚蛋白nlRNA的表达显着低于正常皮肤。结论：这些结果表明，通过胆脂瘤上皮的酸渗漏可能参与了下面骨结构的吸收。胆脂瘤上皮细胞通透性的增加可以通过丝聚蛋白表达的减少来解释。