The mechanism of general anesthesia investigated in the area of non-synaptic neural transmitting at the extracellular space in the brain: focusing on nitric oxide and dopaminergic system

脑细胞外间隙非突触神经传递区域全身麻醉机制研究：关注一氧化氮和多巴胺能系统

基本信息

批准号：
22591706
负责人：
ADACHI Yushi
金额：
$ 2.75万
依托单位：
Nagoya University (2011-2012)Hamamatsu University School of Medicine (2010)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2012
项目状态：
已结题

项目摘要

Pentobarbital (PB) and ketamine (Ket) influence the concentration of neurotransmitters in the brain. PB has been reported to decrease the extracellular nitric oxide (NO) concentration through a decrease in acetylcholine (ACh) release, while Ket has been shown to increase the NO concentration via an increase in ACh release. Here, we investigated effects of PB and Ket on NO release and the relationship between NO and ACh in the rat striatum by in vivo microdialysis experiments. Male Sprague-Dawley rats were used. A microdialysis probe was inserted into the right striatum and perfused with modified Ringer’s solution. Samples were collected every 15 min and injected into an HPLC system. The rats were freely moving, and PB and Ket were administered intraperitoneally. Neostigmine (1 and 10 μM) and mecamylamine (100 μM) were added to the perfusate. Calcium and magnesium concentrations were modified for each anesthetic to influence Ach release. PB decreased NO products (NOx) while Ket increase … More d them. While perfusion with neostigmine showed no effect on baseline Nox concentrations, it diminished the PB-inducedNOx reduction at low concentrations and abolished it at high concentrations. Magnesium-free perfusion had no effect on baseline Nox concentrations, whereas perfusion at a low magnesium concentration antagonized the PB-induced Nox reduction. Mecamylamine and calcium-free perfusion had no effect on baseline Nox concentrations and Ket-induced Nox increases. PB may decrease NO release through reduction in Ach release, whereas Ket may increase NO release independent of Ach regulation.Moreover, we studied a series of preliminary investigations and the results suggested that general anesthetics could modify many pathways of neural transmitting in extracellular space as a non-synaptic neural transmission. Not only GABA_A, which is one of well-known target receptors of anesthetics, but also GABA_Breceptor would be involved in as a target of general anesthetics with the current results of changing in releases of Nox. Less

戊巴比妥（PB）和氯胺酮（KET）会影响大脑中神经递质的浓度。据报道，PB通过乙酰胆碱释放（ACH）释放的降低可降低细胞外一氧化氮（NO）浓度，而KET已证明可以通过增加ACH释放来增加NO浓度。在这里，我们研究了PB和KET对NO释放的影响以及通过体内微透析实验的大鼠纹状体中NO和ACH之间的关系。使用了雄性Sprague-Dawley大鼠。将微透析探针插入右纹状体中，并用改良的铃声溶液灌注。每15分钟收集样品一次，并注入HPLC系统。大鼠自由移动，腹膜内施用PB和KET。在灌注液中添加新生酸（1和10μM）和甲基胺（100μm）。每种麻醉剂都会改变钙和镁浓度，以影响ACH释放。 pb降低了不降低产品（NOX），而KET增加了……更多。虽然新生氨酸灌注对基线NOx浓度没有影响，但它在低浓度下降低了PB诱导的诺克斯降低，并以高浓度消除了它。无镁灌注对基线NOx浓度没有影响，而在低镁浓度下灌注会拮抗PB诱导的NOX的减少。美甲基胺和无钙灌注对基线NOx浓度没有影响，KET诱导的NOX增加。 PB可能通过减少ACH释放而不会释放，而KET可能会增加无独立于ACH调节的释放。此外，我们研究了一系列初步研究，结果表明，全身麻醉药可以改变外细胞外空间中神经传播的许多途径作为非突触神经传播。 GABA_A不仅是麻醉药的众所周知的靶向受体之一，而且GABA_BRECEPTOR也将作为一般麻醉药的目标参与其中，并且当前NOX释放的变化结果。较少的

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Pentobarbital decreased nitric oxide release in the rat striatum but ketamine increased the release independent of cholinergic regulation.

DOI：
10.1538/expanim.61.165
发表时间：
2012
期刊：
Experimental animals
影响因子：
2.4
作者：
K. Kimura-Kuroiwa;Y. Adachi;S. Mimuro;M. Kawamata;Shigehito Sato;N. Matsuda
通讯作者：
K. Kimura-Kuroiwa;Y. Adachi;S. Mimuro;M. Kawamata;Shigehito Sato;N. Matsuda

Dexmedetomidine and hydroxyzine synergistically potentiated the hynotic activity of propofol in ddY mice

右美托咪定和羟嗪协同增强丙泊酚对 ddY 小鼠的催眠活性