Mechanisms of impaired neutrophil functions by clinical isolates from bacteremia

菌血症临床分离株导致中性粒细胞功能受损的机制

• 批准号: 22591116
• 负责人: ATO Manabu
• 金额: $ 2.41万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591116/
• 关键词: 感染症; 免疫学; 細菌; 好中球; 菌血症; 免疫回避; 遺伝子

Sepsis and disseminated bacterial infection is caused by various bacteria and affected to not only compromised hosts but healthy people without apparent trauma. In this study, we aim to unveil a novel neutrophil-bacteria interaction which regulates host defense by analysis of modification of neutrophil defense functions by screening using human neutrophils and mouse models. Here we show that clinical isolates from severe invasive streptococcus infection have a license to kill neutrophils by streptolysin O, a pore-forming toxin in a contact-depending manner. We demonstrate that clinical isolates from septic melioidosis could suppress the release of neutrophil extracellular traps (NETs), which kill bacteria extracellularly. We further identified bacterial virulence factors responsible to modification of NETs release. These findings are expected to contribute to understanding pathogenesis of invasive bacteria infections.

败血症和传播的细菌感染是由各种细菌引起的，不仅受到了宿主的损害，而且还影响了没有明显创伤的健康人。在这项研究中，我们旨在揭示一种新型的中性粒细胞 - 细菌相互作用，该相互作用通过分析使用人类嗜中性粒细胞和小鼠模型筛查中性粒细胞防御功能的修饰来调节宿主防御。在这里，我们表明，来自严重侵入性链球菌感染的临床分离株具有链霉素O杀死嗜中性粒细胞的许可，链霉素O（一种以接触方式进行接触方式形成孔形成毒素。我们证明，来自败血性黑胶质病的临床分离株可以抑制嗜中性粒细胞外陷阱（NET）的释放，这些陷阱会杀死细胞外细菌。我们进一步确定了负责修饰网络释放的细菌毒力因子。这些发现有望有助于理解侵入性细菌感染的发病机理。

项目成果

The defensive role of a novel interferon-γ-producing subpopulation of immature myeloid cells in severe invasive group A Streptococcus infections. 2012

一种新型的未成熟骨髓细胞产生干扰素亚群在严重侵袭性 A 族链球菌感染中的防御作用 2012。

• 发表时间: 2013
• 作者: Matsumura T;Ikebe T;Ohnishi M;Watanabe H;Kobayashi K;Ato M
• 通讯作者: Ato M

novel IFN-g-producing subpopulations of immature myeloid cells confers protection from severe invasive group A Streptococcus infections. 2011.

未成熟骨髓细胞中新的产生 IFN-g 的亚群可防止严重侵袭性 A 族链球菌感染。

• 发表时间: 2011
• 作者: Matsumura T;Ikebe T;Ohnishi M;Watanabe H;Kobayashi K;Ato M
• 通讯作者: Ato M

Grobal threads and the control of multi-drug resistant tuberculosis.

全球线索和耐多药结核病的控制。

• 发表时间: 2011
• 期刊: Journal of Disaster Research
• 影响因子: 0.8
• 作者: Kobayashi K;Ato M;Matsumoto S
• 通讯作者: Matsumoto S

The defensive role of a novel interferon-γ-producing subpopulation of immature myeloid cells in severe invasive group A Streptococcus infections.

一种新型的未成熟骨髓细胞产生干扰素 γ 的亚群在严重侵袭性 A 族链球菌感染中的防御作用。

• 发表时间: 2012
• 作者: Matsumura T;Ikebe T;Ohnishi M;Watanabe H;Kobayashi K;Ato M.
• 通讯作者: Ato M.

劇症型溶血性レンサ球菌感染症における菌側病原因子と好中球機能傷害の機序

暴发性溶血性链球菌感染中性粒细胞功能障碍的细菌致病因素及机制

• 发表时间: 2013
• 期刊: 化学療法の領域
• 作者: 阿戸 学;松村隆之;池辺忠義
• 通讯作者: 池辺忠義