Elucidation of the mechanisms involved in development and suppression ofBarrett's esophagus: Results of joint Japan-U.S. research

阐明巴雷特食管的发育和抑制机制：日美联合研究的结果

• 批准号: 22590692
• 负责人: WATARI Jiro
• 金额: $ 2.75万
• 依托单位: Hyogo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590692/
• 关键词: 上部消化管学(食道,胃,十二指腸); バレット食道; 特殊腸上皮化生; 円柱上皮化生; プロトンポンプ阻害薬; メチル化異常; マイクロサテライト不安定性; Das-1抗体; ゲノム不安定性; Helicobacter pylori

We evaluated several molecular alterations including microsatellite instability (MSI); the CpG island methylation status of hMLH1, p16, E-cadherin and APC genes; and monoclonal antibody Das-1, which specifically reacts with BE in specialized intestinal metaplasia (SIM), as well as in columnar lined epithelium (CLE) without SIM in BE mucosa. Additionally, we investigated changes in the above molecular markers in a randomized control trial, comparing PPI administration versus PPI non-administration groups. Our results showed no significant differences in the frequency of MSI, or methylation at any genes investigated in SIM or CLE. In contrast, Das-1 reactivity was significantly higher in SIM versus CLE. The frequency of methylation and Das-1 reactivity among Japanese was similar to that of BE in the U.S. population, a finding which corroborates previous reports. In patients administered PPI, MSI and hypermethylation at p16, E-cadherin, and APC genes disappeared following interventon, while patients without PPI did not show this tendency. These results indicate that CLE may be a precancerous lesion as well as SIM, and there is no difference in the molecular characteristics of BE between Japanese and U.S. populations. Furthermore, PPI may promote regression of the molecular alterations involved in BE.

我们评估了包括微卫星不稳定性（MSI）在内的几个分子改变； HMLH1，P16，E-钙粘蛋白和APC基因的CpG岛甲基化状态；和单克隆抗体DAS-1，这些抗体与专门的肠道化生（SIM）以及在没有SIM的柱状上皮（CLE）中专门反应。此外，我们在随机对照试验中研究了上述分子标记的变化，比较了PPI给药与PPI非管理组的变化。我们的结果表明，在SIM或CLE研究的任何基因上，MSI的频率或甲基化没有显着差异。相反，SIM与CLE中的DAS-1反应性明显更高。日本人之间甲基化和DAS-1反应性的频率与美国人群的BE相似，这一发现证实了先前的报告。在介入后，在P16，E-钙粘着蛋白和APC基因的患者中，PPI，MSI和高甲基化消失了，而没有PPI的患者没有显示这种趋势。这些结果表明，CLE可能是癌前病变和SIM，并且日本人和美国人群之间BE的分子特征没有差异。此外，PPI可能会促进BE中涉及的分子改变的回归。

项目成果

肥満と消化器疾患

肥胖和消化系统疾病

• 发表时间: 2010
• 作者: Sato T;Takimoto R;小池和彦(共著)
• 通讯作者: 小池和彦(共著)

Molecular alterations, the cellular phenotype and proliferation in Barrett's esophagus in a Japanese population with H. pylori infection

幽门螺杆菌感染的日本人群巴雷特食管的分子改变、细胞表型和增殖

• 发表时间: 2010
• 作者: Watari J;Moriichi K;Tanabe H;et. al.
• 通讯作者: et. al.

A pseudosarcomatous lesion resembling a malignant tumor of the esophagocardiac junction, diagnosed by a total biopsy with endoscopic surgery

类似食管贲门交界处恶性肿瘤的假肉瘤病变，通过内窥镜手术进行全活检诊断

• DOI: 10.1055/s-0031-1291502
• 发表时间: 2012
• 期刊: Endoscopy
• 影响因子: 9.3
• 作者: Ando K;Fujiya M;Ito T;Watari J;et al
• 通讯作者: et al

Autofluorescence imaging and the quantitative intensity of fluorescence for evaluating the dysplastic grade of colonic neoplasms

自发荧光成像和荧光定量强度评估结肠肿瘤的发育不良级别

• 发表时间: 2012
• 期刊: Int J Colorectal Diseases
• 作者: Moriichi K;Fujiya M;et al.
• 通讯作者: et al.

Morphological changes of colonic Dieulafoy's lesion : a case that could be retrospectively reviewed in a patient without treatment

结肠迪乌拉福伊病灶的形态学变化：一例未经治疗的患者可以回顾性观察的病例

• DOI: 10.1007/s12328-011-0254-5
• 发表时间: 2011
• 期刊: Clin J Gastroenterol
• 影响因子: 1
• 作者: Watari J;Yamasaki T;Kondo T;et al
• 通讯作者: et al