Elucidation of the mechanisms involved in development and suppression ofBarrett's esophagus: Results of joint Japan-U.S. research

阐明巴雷特食管的发育和抑制机制：日美联合研究的结果

• 批准号: 22590692
• 负责人: WATARI Jiro
• 金额: $ 2.75万
• 依托单位: Hyogo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590692/
• 关键词: 上部消化管学(食道,胃,十二指腸); バレット食道; 特殊腸上皮化生; 円柱上皮化生; プロトンポンプ阻害薬; メチル化異常; マイクロサテライト不安定性; Das-1抗体; ゲノム不安定性; Helicobacter pylori

We evaluated several molecular alterations including microsatellite instability (MSI); the CpG island methylation status of hMLH1, p16, E-cadherin and APC genes; and monoclonal antibody Das-1, which specifically reacts with BE in specialized intestinal metaplasia (SIM), as well as in columnar lined epithelium (CLE) without SIM in BE mucosa. Additionally, we investigated changes in the above molecular markers in a randomized control trial, comparing PPI administration versus PPI non-administration groups. Our results showed no significant differences in the frequency of MSI, or methylation at any genes investigated in SIM or CLE. In contrast, Das-1 reactivity was significantly higher in SIM versus CLE. The frequency of methylation and Das-1 reactivity among Japanese was similar to that of BE in the U.S. population, a finding which corroborates previous reports. In patients administered PPI, MSI and hypermethylation at p16, E-cadherin, and APC genes disappeared following interventon, while patients without PPI did not show this tendency. These results indicate that CLE may be a precancerous lesion as well as SIM, and there is no difference in the molecular characteristics of BE between Japanese and U.S. populations. Furthermore, PPI may promote regression of the molecular alterations involved in BE.

我们评估了几种分子改变，包括微卫星不稳定性（MSI）； hMLH1、p16、E-钙粘蛋白和 APC 基因的 CpG 岛甲基化状态；单克隆抗体 Das-1，它与特化肠化生 (SIM) 中的 BE 以及 BE 粘膜中无 SIM 的柱状衬里上皮 (CLE) 中的 BE 发生特异性反应。此外，我们在一项随机对照试验中研究了上述分子标志物的变化，比较了 PPI 给药组和未给药组。我们的结果显示，SIM 或 CLE 中研究的任何基因的 MSI 频率或甲基化没有显着差异。相比之下，SIM 中的 Das-1 反应性明显高于 CLE。日本人的甲基化和 Das-1 反应性频率与美国人群中的 BE 相似，这一发现证实了之前的报告。在接受 PPI 的患者中，MSI 和 p16 的高甲基化、E-钙粘蛋白和 APC 基因在干预后消失，而未接受 PPI 的患者则没有表现出这种趋势。这些结果表明，CLE可能与SIM一样是癌前病变，并且日本和美国人群之间BE的分子特征没有差异。此外，PPI 可能会促进 BE 相关分子改变的消退。

项目成果

肥満と消化器疾患

肥胖和消化系统疾病

• 发表时间: 2010
• 作者: Sato T;Takimoto R;小池和彦(共著)
• 通讯作者: 小池和彦(共著)

Molecular alterations, the cellular phenotype and proliferation in Barrett's esophagus in a Japanese population with H. pylori infection

幽门螺杆菌感染的日本人群巴雷特食管的分子改变、细胞表型和增殖

• 发表时间: 2010
• 作者: Watari J;Moriichi K;Tanabe H;et. al.
• 通讯作者: et. al.

A pseudosarcomatous lesion resembling a malignant tumor of the esophagocardiac junction, diagnosed by a total biopsy with endoscopic surgery

类似食管贲门交界处恶性肿瘤的假肉瘤病变，通过内窥镜手术进行全活检诊断

• DOI: 10.1055/s-0031-1291502
• 发表时间: 2012
• 期刊: Endoscopy
• 影响因子: 9.3
• 作者: Ando K;Fujiya M;Ito T;Watari J;et al
• 通讯作者: et al

Autofluorescence imaging and the quantitative intensity of fluorescence for evaluating the dysplastic grade of colonic neoplasms

自发荧光成像和荧光定量强度评估结肠肿瘤的发育不良级别

• 发表时间: 2012
• 期刊: Int J Colorectal Diseases
• 作者: Moriichi K;Fujiya M;et al.
• 通讯作者: et al.

わが国のGERDとバレット食道の関わり

日本GERD与Barrett食管的关系

• 发表时间: 2012
• 期刊: THEGIFOREFRONT
• 作者: 渡二郎;富田寿彦;大島忠之;他
• 通讯作者: 他