Studies on Helicobacter species on gastric MALT lymphoma and effect of molecular target drugs

幽门螺杆菌对胃MALT淋巴瘤的影响及分子靶向药物的研究

基本信息

批准号：
22590690
负责人：
NAKAMURA Masahiko
金额：
$ 2.91万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2012
项目状态：
已结题

项目摘要

By the investigation of the specimens obtained from the upper gastrointestinal endoscopy of the patients suffering from chronic gastritis, gastric ulcer, gastric polyp, gastric cancer and gastric MALT lymphoma, 7 MALT lymphoma cases out of 18 (38%) were found to be positive to Helicobacter heilmannii, while only 2% were positive in other gastric diseases. By the cluster analysis using GENENTYC-MAC UPGMA, these cases were found to be infected by Helicobacter heilmannii and suis. Among other gastric diseases, nodular gastritis patents were found to be highly positive to H. heilmannii, suggesting the possible interaction with the gastric cancer. In H. heilmannii positive cases, more than half cases were found to show the positivity to H. pylori as well.By the in vivo study using the infection model using C57BL/6 mice, VEGF-related immunoreactities were recognized in the MALT lymphoma, suggesting the VEGF-mediated angiogenesis was closely related to the expansion of the tumor. The administration of Flt-4 and Flt-1 antibody suppressed the tumor, supporting this hypothesis. c-Met immunoreactivity was found in the lymphocytes composing the MALT lymphoma, and HGF immunoreactivity was recognized mostly in the endothelial cells and macrophages. HGFA was localized on mesenchymal cells other than the lymphocytes. The administration of antibodies against c-Met to the infected mice induced the significant suppression of hepatic and pulmonary lesions as well as the gastric MALT lymphoma.In conclusion, HGF and c-Met pathway were suggested to contribute to the lymphomagenesis in the liver and lung after Helicobacter heilmannii infection.

通过研究从慢性胃炎，胃溃疡，胃溃疡，胃息肉，胃癌和胃癌淋巴瘤的患者的上胃肠道内窥镜获得的标本，发现18例麦芽淋巴瘤的7例（38％）在Heilmannii heilmannii阳性中呈阳性，而另外2％的症状是阳性的。通过使用GenenteC-MAC UPGMA的聚类分析，发现这些病例被Heilmannii和Suis感染。在其他胃疾病中，发现结节性胃炎专利对H. Heilmannii高度阳性，这表明可能与胃癌存在相互作用。在H. heilmannii阳性病例中，发现了一半以上的病例表明对幽门螺杆菌的阳性。通过使用C57BL/6小鼠的感染模型进行的体内研究，与VEGF相关的免疫反应性在麦芽瘤中识别出与VEGGF介导的血管生成相关的麦芽瘤，表明是在麦芽瘤中识别的。 FLT-4和FLT-1抗体的施用抑制了肿瘤，支持了这一假设。在组成麦芽淋巴瘤的淋巴细胞中发现了C-MET免疫反应性，HGF免疫反应性主要在内皮细胞和巨噬细胞中被识别。 HGFA位于淋巴细胞以外的间充质细胞上。对感染小鼠的C-MET抗体施用肝和肺部病变的显着抑制以及胃麦芽淋巴瘤的显着抑制。在最后，建议HGF和C-MET途径有助于HELICBACTER HEILMANNII IFTECTION后肝脏和肺部的淋巴瘤发生。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Suppression of lymphangiogenesis induced by Flt-4 antibody in gastric low-grade mucosa-associated lymphoid tissue lymphoma by Helicobacter heilmannii infection

DOI：
10.1111/j.1440-1746.2010.06230.x
发表时间：
2010-05-01
期刊：
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
影响因子：
4.1
作者：
Nakamura, Masahiko;Matsui, Hidenori;Tsuchimoto, Kanji
通讯作者：
Tsuchimoto, Kanji

胃リンパ腫をターゲットとした各種モノクローナル抗体による抗腫瘍効果について。シンポジウム4消化管疾患の病態生理と治療薬の開発

关于各种针对胃淋巴瘤的单克隆抗体的抗肿瘤作用。

DOI：
发表时间：
2011
期刊：
影响因子：
0
作者：
Nakamura;M.;中村正彦
通讯作者：
中村正彦

Helicobacter heilmannii can induce gastric lymphoid follicles in mice via a Peyer's patch-independent pathway

DOI：
10.1111/j.1574-695x.2010.00731.x
发表时间：
2010-11-01
期刊：
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
影响因子：
0
作者：
Nobutani, Kentaro;Yoshida, Masaru;Azuma, Takeshi
通讯作者：
Azuma, Takeshi

胃、肝、肺MALTリンパ腫形成におけるc-Met, Hepatocyte Growth Factorの関与:c-Met抗体による腫瘍縮小効果

c-Met 和肝细胞生长因子参与胃、肝和肺 MALT 淋巴瘤形成：c-Met 抗体的肿瘤减少作用

DOI：
发表时间：
2012
期刊：
Prog Med
影响因子：
0
作者：
横山展子; 中村正彦;松田唯;馬庭優子;伊香萌菜;松井英則;高橋哲史;高橋信一;土本寛二
通讯作者：
土本寛二

Angiogenesis and Lymphangiogenesis in Malignancy: Lesson from Gastric MALT Lymphoma Model

恶性肿瘤中的血管生成和淋巴管生成：胃 MALT 淋巴瘤模型的教训

DOI：
发表时间：
2013
期刊：
Current Medicinal Chemistry
影响因子：
4.1
作者：
Fukui H;Zhang X;Sun C;Yamasaki T;Kondo T;Ikehara H;Tomita T;Oshima T;横山展子;Masahiko Nakamura
通讯作者：
Masahiko Nakamura