Construction of molecular basis and clinical application of Kampo medicine

汉方医学的分子基础构建及临床应用

基本信息

批准号：
22590666
负责人：
ARAI Makoto
金额：
$ 2.83万
依托单位：
Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2010
资助国家：
日本
起止时间：
2010-04-01 至 2014-03-31
项目状态：
已结题

项目摘要

Kampo crude drug, daio (Rhei rhizoma) is frequently used as a purgative for a long time. We previously reported the extending life span of nematode treated with daio, however, its biological response by administration of daio remains unclear. In this study, to clarify the molecular mechanisms on life extension by daio in nematode, we screened the genes upregulated by administration of daio using microarray analysis. We obtained six genes transcriptionally activated more than 10 times by daio and their mammalian homologues were characterized in mice. By 2-week oral administration, expression of Nhe1, one of the six candidate genes, in kidney was upregulated similarly to that in nematode. These findings suggest that the Nhe1 encoding Na+/H+ exchanger protein plays an important role in maintaining kidney structurally and functionally.To use daio in animal experiments, we have also developed a method for preparing sennoside-reduced daio, which maintains most of other major components.

坎波原油，daio（Rhei Rhizoma）很长时间以来经常用作泻药。我们先前报道了用Daio处理的线虫的延长寿命，但是，其通过给药的生物学反应仍然不清楚。在这项研究中，为了阐明Daio在线虫中的生命延伸的分子机制，我们使用微阵列分析筛选了通过DAIO上调的基因。我们获得了Daio的六个转录激活的基因，其哺乳动物的同源物在小鼠中的表征。到2周的口服给药，NHE1（六个候选基因之一）在肾脏中的表达与线虫中的表达相似。这些发现表明，编码Na+/H+交换器蛋白的NHE1在结构和功能上保持肾脏起着重要作用。要在动物实验中使用DAIO，我们还开发了一种准备降低塞纳西德的DAIO的方法，该方法维持大多数其他主要成分。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Xanthones and a benzophenone from the roots of Pentadesma butyracea and their antiproliferative activity

DOI：
10.1016/j.phytol.2010.02.006
发表时间：
2010-06
期刊：
Phytochemistry Letters
影响因子：
1.7
作者：
H. K. Wabo;H. Kikuchi;Yasuhiro Katou;P. Tane;Y. Oshima
通讯作者：
H. K. Wabo;H. Kikuchi;Yasuhiro Katou;P. Tane;Y. Oshima

Phenolic Compounds and Terpenoids from Hypericum lanceolatum

DOI：
发表时间：
2012
期刊：
Records of Natural Products
影响因子：
1.9
作者：
H. K. Wabo;T. Kowa;A. H. Lonfouo-;Alembert Tchinda Tiabou;P. Tane;H. Kikuchi;M. Frédérich;Y. Oshima
通讯作者：
H. K. Wabo;T. Kowa;A. H. Lonfouo-;Alembert Tchinda Tiabou;P. Tane;H. Kikuchi;M. Frédérich;Y. Oshima

消化器疾患と漢方治療

消化系统疾病及中草药治疗