Biological activities of ovine and bovine placental prolactin family proteins

绵羊和牛胎盘催乳素家族蛋白的生物活性

• 批准号: 22580327
• 负责人: TAKAHASHI Toru
• 金额: $ 2.91万
• 依托单位: National Institute of Agrobiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580327/
• 关键词: ウシ; 胎盤; プロラクチン; カテプシン; 血管内皮細胞; MMP; 7S; IV型コラーゲン

Bovine prolactin-related protein-I (PRP1) is a non-classical member of prolactin (PRL) family proteins. However, its function is still unknown. PRL, when cleaved by cathepsin D and matrix metalloproteinases (MMPs), resulted in N-terminal 16 kDa fragments (16K-PRL) that have antiangiogenetic properties in human and rodents. We examined the possibility of similar activity of bovine PRP1. PRP1 (normally 33 kDa) was cleaved by cathepsins (CTSs), MMPs, and bovine cotyledonary-conditioned medium (BCCM), and generated mainly 26 kDa N-terminal fragments. Two specific enzyme families, CTSs and MMPs cleaved intact PRP1, and BCCM also contained PRP1 cleavage activity. Bioactivity of cleaved PRP1 was examined in a cell proliferation assay using bovine brain vascular endothelial cells. The cleaved PRP1 stimulated the proliferation of endothelial cells in vitro. The endothelial cell proliferation activity of cleaved PRP1 may be shared in specific bovine placentomal angiogenesis.

牛催乳素相关蛋白I（PRP1）是催乳素（PRL）家族蛋白的非经典成员。但是，其功能仍然未知。 PRL被组织蛋白酶D和基质金属蛋白酶（MMP）裂解时，导致N末端16 kDa片段（16K-PRL）在人和啮齿动物中具有抗静脉发育特性。我们检查了牛PRP1类似活性的可能性。 PRP1（通常为33 kDa）被组织蛋白酶（CTSS），MMPS和牛共叶调节培养基（BCCM）裂解，并主要产生26 kDa N末端片段。两个特定的酶家族，CTS和MMP裂解完整的PRP1，而BCCM也包含PRP1裂解活性。使用牛脑血管内皮细胞在细胞增殖测定中检查了裂解PRP1的生物活性。切割的PRP1在体外刺激了内皮细胞的增殖。裂解PRP1的内皮细胞增殖活性可以在特定的牛胎盘血管生成中共享。

项目成果

Cleaved bovine prolactin-related protein-I stimulates vascular endothelial cell proliferation

• DOI: 10.1016/j.mce.2010.03.008
• 发表时间: 2010-07-29
• 期刊: MOLECULAR AND CELLULAR ENDOCRINOLOGY
• 影响因子: 4.1
• 作者: Ushizawa, Koichi;Takahashi, Toru;Hashizume, Kazuyoshi
• 通讯作者: Hashizume, Kazuyoshi

Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and its related extracellular matrix degrading enzymes in the endometrium during estrous cycle and early gestation in cattle

• DOI: 10.1186/1477-7827-8-60
• 发表时间: 2010-06-11
• 期刊: REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
• 影响因子: 4.4
• 作者: Mishra, Birendra;Kizaki, Keiichiro;Hashizume, Kazuyoshi
• 通讯作者: Hashizume, Kazuyoshi

A novel factor for the regulation of utero-placental angiogenesis

调节子宫胎盘血管生成的新因子

• 发表时间: 2010
• 作者: Takahashi T;Ushizawa K;Hosoe M;Kizaki K;Hashizume K
• 通讯作者: Hashizume K

ウシ抹消白血球に発現する妊娠シグナル

牛外周白细胞表达的妊娠信号

• 发表时间: 2013
• 作者: 橋爪一善;木崎あゆみ;茂野智子;木崎景一郎;松田秀雄;橋谷田 豊;今井 敬;古澤 軌;細江実佐
• 通讯作者: 細江実佐

Anovel factor for the regulation of utero-placental angiogenesis

调节子宫胎盘血管生成的新因子

• 发表时间: 2010
• 作者: Takahashi T;Ushizawa K;Hosoe M;Kizaki K;Hashizume K
• 通讯作者: Hashizume K