Detection and analysis of invasive insect populations based on mtDNA sequences of stored and newly collected specimens

基于储存和新采集标本的线粒体DNA序列检测和分析入侵昆虫种群

基本信息

批准号：
22580061
负责人：
MURAJI Masahiko
金额：
$ 2.91万
依托单位：
National Institute of Agrobiological Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2012
项目状态：
已结题

项目摘要

Genetic variation of a Japanese longicorn beetle Anoplophora spp. was examined based on mitochondrial DNA sequences. Using stored specimens and newly collected samples obtained from 75 localities in the Japanese mainland, the Ryukyu Islands, and Taiwan, two fragments, a 1.2 kb-long fragment containing the cytochrome oxidase genes, and a 1.4 kb-long one containing rDNAs, were sequenced. In phylogenetic analyses based on the sequences, 294 individuals were divided into two major groups and then split into seven subgroups. In Group A, the closely related subgroups A1, A2, and A3 were mainly distributed on the Japanese mainland and were roughly separated among geographic areas, although the range of A3 spread from Kyushu to the Central Ryukyu Islands. The sequence of A4, detected from one individual collected in eastern Honshu, was the same as that of Anoplophora on the Chinese continent. In Group B, subgroups B1 and B2 were restricted to the Central and Southern Ryukyu Islands, respectively, while B3 was distributed widely in both regions. These results suggested that local populations of this beetle were disturved by insect movements including those caused by human activities.

日本天牛星天牛的遗传变异。根据线粒体 DNA 序列进行检查。利用保存的标本和从日本本土、琉球群岛和台湾的75个地方获得的新采集的样本，对两个片段进行了测序，一个是包含细胞色素氧化酶基因的1.2 kb长片段，另一个是包含rDNA的1.4 kb长片段。在基于序列的系统发育分析中，294 个人被分为两个主要组，然后又分为七个亚组。 A组中，关系密切的A1、A2、A3亚组主要分布在日本本土，地理区域大致分离，但A3的范围从九州延伸至琉球群岛中部。在本州岛东部采集的一个个体中检测到的A4序列与中国大陆的星天牛的序列相同。 B组中，B1和B2亚组分别仅限于琉球群岛中部和南部，而B3则广泛分布于这两个地区。这些结果表明，这种甲虫的当地种群受到昆虫运动（包括人类活动引起的昆虫运动）的干扰。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Genetic variation of the whitespotted longicorn beetle Anoplophora spp. (Coleoptera: Cerambycidae) in Japan detected by mitochondrial DNA sequence

白斑天牛甲虫星天牛的遗传变异。

DOI：
10.1007/s13355-011-0056-8
发表时间：
2011
期刊：
Applied Entomology and Zoology
影响因子：
1.3
作者：
M. Muraji;S. Wakamura;H. Yasui;N. Arakaki;Y. Sadoyama;S. Ohno and K. Matsuhira
通讯作者：
S. Ohno and K. Matsuhira

石垣島において発生時期が異なるケブカアカチャコガネの分布と遺伝子解析

石垣岛不同爆发时期红斑甲虫的分布及遗传分析