Role of a calcium-binding protein in the saliva of Nephotettix cincticeps in ingestion from the sieve tubes

黑尾叶蝉唾液中钙结合蛋白在筛管摄入中的作用

• 批准号: 22580063
• 负责人: HATTORI Makoto
• 金额: $ 2.91万
• 依托单位: National Institute of Agrobiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580063/
• 关键词: 害虫管理; 唾液; EF-ハンドモチーフ; カルシウム結合タンパク質; 師管吸汁; ツマグロヨコバイ

Green rice leafhoppers (Nephotettix cincticeps) secrete saliva during feeding. In this study, we found an 84 kDa calcium-binding protein (NcSP84) in the saliva. The NcSP84 was produced in the largest lobes in the primary salivary glands. The NcSP84 was detected in the phloem sap of rice exposed to leafhoppers, verifying that the NcSP84 protein was injected into the sieve tubes. The nucleotide and amino acid sequences of NcSP84 did not share homology with any sequences in public databases. Motif search predicted that this protein had multiple EF-hands, the most common motif found in Ca^<2+>-binding proteins. As predicted, NcSP84 exhibited Ca^<2+>-binding activity. The mobility of purified NcSP84 bound to Ca^<2+> declined during SDS-PAGE, depending on the concentration of CaCl_2 present. These results suggest that NcSP84 could be secreted into the sieve tubes during feeding, which might bind Ca^<2+> ions that flow into sieve tubes in response to stylet puncturing. This might suppress sieve-element clogging and facilitate continuous ingestion from sieve tubes. This is the first finding of gene cloning of an EF-hand Ca^<2+>-binding protein in the saliva of a vascular-feeding insect

喂食期间，绿色稻叶虫（Nephotettix cincticeps）分泌唾液。在这项研究中，我们在唾液中发现了84 kDa钙结合蛋白（NCSP84）。 NCSP84是在初级唾液腺中最大的叶片中产生的。在暴露于叶霍普珀的水稻的韧皮部汁液中检测到了NCSP84，并验证了将NCSP84蛋白注入筛管中。 NCSP84的核苷酸和氨基酸序列与公共数据库中的任何序列共享同源性。基元搜索预测该蛋白具有多个EF手，这是Ca^<2+> - 结合蛋白中最常见的基序。如预测的，NCSP84表现出CA^<2+> - 结合活性。在SDS-PAGE期间，纯化的NCSP84的迁移率取决于CACL_2的浓度。这些结果表明，NCSP84可以在喂食过程中分泌到筛管中，后者可能会结合ca^<2+>离子，这些离子以响应于骨的刺穿而流入筛子中。这可能会抑制筛子堵塞并促进筛子的连续摄入。这是Ef手Ca^<2+>的基因克隆的第一个发现，在血管喂养昆虫的唾液中

项目成果

Molecular cloning of a novel calcium-binding protein in the secreted saliva of the green rice leafhopper

绿稻叶蝉分泌唾液中新型钙结合蛋白的分子克隆

• 发表时间: 2012
• 期刊: Nephotettix cincticeps Insect Biochemistry and Molecular Biology
• 作者: Hattori M;Nakamura M;Komatsu S;Tsuchihara K;Tamura Y;Hasegawa T
• 通讯作者: Hasegawa T

長谷川毅, 服部誠, 野田博明

长谷川武、服部诚、野田宏明

• 发表时间: 2013
• 作者: 長谷川毅;服部誠;野田博明
• 通讯作者: 野田博明

A Calcium-binding and other functional proteins in the saliva of the green rice leafhopper

A 绿稻叶蝉唾液中的钙结合蛋白和其他功能蛋白

• 发表时间: 2012
• 作者: Hattori M;Komatsu S;Nakamura M;Tamura Y
• 通讯作者: Tamura Y

Molecular cloning of a novel calcium-binding protein in the secreted saliva of the green rice leafhopper Nephotettix cincticeps

• DOI: 10.1016/j.ibmb.2011.10.001
• 发表时间: 2012-01-01
• 期刊: INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
• 影响因子: 3.8
• 作者: Hattori, Makoto;Nakamura, Masatoshi;Hasegawa, Tsuyoshi
• 通讯作者: Hasegawa, Tsuyoshi

A calcium-binding and other functional proteins in the saliva of the green rice leafhopper, Nephotettix cincticeps

绿叶蝉 (Nephotettix cincticeps) 唾液中的钙结合蛋白和其他功能蛋白

• 发表时间: 2012
• 作者: Hattori;M.;Komatsu;S.;Nakamura,M.;Tamura;Y.
• 通讯作者: Y.