Analysis on the function of alleles of good grain filling contributing to the improvement of grain filling ability of rice extra-heavy panicle types

灌浆好等位基因对提高水稻特重穗型灌浆能力的作用分析

• 批准号: 22580020
• 负责人: KATO Tsuneo
• 金额: $ 2.75万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580020/
• 关键词: イネ; 極穂重型品種; 登熟; ADPグルコースピロホスホリラーゼ; ショ糖トランスポーター; 塩基配列; イネ極穂重型品種; ADPグルコースピロホスホリラーゼ大サブユニット; ADPグルコースピロホスホリラーゼ小サブユニット; 分子マーカー; 塩基配列多型; 対立遺伝子; 定量PCR; 多重PCR; 粒重増加過程

Alleles for good grain filling contributing to the improvement of poor grain filling in extra-heavy panicle types of rice were explored. As a result, APS2-2, APL2-2 and SUT1-1 at the locus for ADPglucose pyrophosphorylase (AGPase) small subunit 2 (OsAGPS2), large subunit 2 (OsAGPL2) and sucrose transporter 1 (OsSUT1), respectively, were detected as candidate alleles. The cultivars with all of these three alleles showed higher AGPase activity in developing endosperm, and higher degree of grain filling at maturity. Among these three alleles, APL2-2 might display the highest effect on grain filling. These three alleles tended to be included together in indica cultivars. Therefore, alleles for good grain filling should be searched among indica rice cultivars to serve the poor grain filling problems. However, these alleles could not contribute directly to higher yield.

研究了有助于改善超重穗型水稻籽粒灌浆不良的籽粒灌浆良好等位基因。 结果，ADP葡萄糖焦磷酸化酶（AGPase）小亚基2（OsAGPS2）、大亚基2（OsAGPL2）和蔗糖转运蛋白1（OsSUT1）基因座上的APS2-2、APL2-2和SUT1-1分别被检测为候选等位基因。 具有所有这三个等位基因的品种在发育中的胚乳中表现出更高的AGP酶活性，并且在成熟时籽粒灌浆程度更高。 在这三个等位基因中，APL2-2 可能对籽粒灌浆表现出最高的影响。 这三个等位基因往往一起包含在籼稻品种中。 因此，应在籼稻品种中寻找灌浆良好的等位基因，以解决灌浆不良的问题。 然而，这些等位基因不能直接有助于提高产量。

项目成果

A novel frameshift mutant allele, fzp-10, affecting the panicle architecture of rice

• DOI: 10.1007/s10681-011-0547-2
• 发表时间: 2012-03-01
• 期刊: EUPHYTICA
• 影响因子: 1.9
• 作者: Kato, Tsuneo;Horibata, Akira
• 通讯作者: Horibata, Akira

Effects of the alleles at OsAGPS2 and OsSUT1 on the grain filling in extra-heavy panicle type of rice

OsAGPS2和OsSUT1等位基因对超重穗型水稻籽粒灌浆的影响

• DOI: 10.2135/cropsci2009.11.0690
• 发表时间: 2010
• 期刊: Crop Science
• 影响因子: 2.3
• 作者: Kato;T.;Taniguchi;A.;Horibata;A.
• 通讯作者: A.

A trial to develop novel genotypes of extra-heavy panicle types with good grain filling in rice

水稻灌浆性特重穗型新基因型的开发试验

• 发表时间: 2013
• 期刊: 作物研究
• 作者: Kato;T.
• 通讯作者: T.

イネ組換え近交系の出穂後の稈におけるデンプン含量およびデンプン代謝関連遺伝子発現量の推移

水稻重组自交系抽穗后秆中淀粉含量及淀粉代谢相关基因表达量的变化

• 发表时间: 2013
• 作者: Kato;T.;Taniguchi;A.;Horibata;A.;久保竜一,堀端章,加藤恒雄
• 通讯作者: 久保竜一,堀端章,加藤恒雄

Non-random distribution of the alleles for good grain filling at OsAGPS2 and OsSUT1 among a wide range of rice (Oryza sativa L.) cultivars

• DOI: 10.1270/jsbbs.61.217
• 发表时间: 2011-06-01
• 期刊: BREEDING SCIENCE
• 影响因子: 2.4
• 作者: Kato, Tsuneo;Horibata, Akira
• 通讯作者: Horibata, Akira