Study on the mechanism of anthocyanin biosynthesis and flower color breeding of Dutch iris

荷兰鸢尾花色苷生物合成及花色育种机制研究

• 批准号: 22580007
• 负责人: YABUYA Tsutomu
• 金额: $ 2.91万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580007/
• 关键词: ダッチアイリス; 5GT 遺伝子; アントシアニン; フラボン; アントシアニン生合成遺伝子; CHS 遺伝子; ペチュニア形質転換体; 外花被の白色化; CHS遺伝子; 5GT遺伝子; アグロバクテリウム法; ペチュニア形質転換系統; DFR遺伝子; ポリアシル化; p-クマル酸; マロン酸; ゲノム遺伝子; CHI遺伝子; F3'5'H遺伝子

In this study, to promote flower color breeding of the Iris garden species, anthocyanins, flavones and anthocyanin biosynthetic genes of Dutch iris (Iris x hollandica) cultivars were characterized. Malonate anthocyanins of delphinidin3pCRG5G, petunidin 3pCRG5G, malvidin3pCRG5G and acetylated C-glycosyl flavones were pre- sumed as novel pigments in the genus Iris. As anthocyanin biosynthetic genes, full length of DFR genes (gDNA) and cDNA homologues of 3RT、F3’5’H, CHI genes were isolated. In addition, transgenic petunia lines with CHS or 5GT genes isolated from the cultivar, ‘Blue Diamond’ were obtained by Agrobacterium methods and were characterized. Finally, the defect or reduction of DFR gene expression in outer perianths of the cultivars ‘White Wedgewood’ and ‘Surprise’ were presumed to be caused by insertions LTR-like retrotransposons into promoter regions of their DFR genes.

在这项研究中，为了促进鸢尾品种的花色育种，对荷兰鸢尾（Iris x hollandica）品种的花青素、黄酮和花青素生物合成基因进行了表征，包括花翠素3pCRG5G、矮牵牛素3pCRG5G、锦葵素3pCRG5G和乙酰化C-糖基黄酮。假定的作为花青素生物合成基因，分离了全长DFR基因(gDNA)和3RT、F3'5'H、CHI基因的cDNA同源物。此外，还分离了具有CHS或5GT基因的转基因矮牵牛品系。通过农杆菌方法从 Cult theivar 中获得“Blue Diamond”，并对其外花被中 DFR 基因表达的缺陷或减少进行了表征。据推测，“White Wedgewood”和“Surprise”品种的突变是由于将 LTR 样反转录转座子插入其 DFR 基因的启动子区域引起的。

项目成果

ナスヒオウギアヤメおよびキリガミネヒオウギアヤメの起源

那须鸢尾和雾峰鸢尾的起源

• 发表时间: 2012
• 作者: 藪谷 勤;花崎 歩;冨田武志;井上公一
• 通讯作者: 井上公一

二倍体ヒオウギアヤメと二倍体カキツバタの種間交雑による異質三倍体の獲得

二倍体虹膜与二倍体虹膜种间杂交获得同种异体三倍体

• 发表时间: 2011
• 作者: 藪谷 勤;花崎 歩;冨田武志;井上公一;Takayuki Mizuno;水野貴行;藪谷勤;藪谷 勤;藪谷勤;藪谷勤
• 通讯作者: 藪谷勤

Molecular characterization of cDNA clones encoding flavanone 3-hydroxylase from Dutch iris (Iris x hollandica)

荷兰鸢尾 (Iris x hollandica) 黄烷酮 3-羟化酶 cDNA 克隆的分子表征

• 发表时间: 2012
• 期刊: Cytologia
• 影响因子: 1
• 作者: Mizuno Takaaki;Yabuya Tsutomu;Sasaki Nobuhiro;Iwashina Tsukasa;Noriko Yoshihara
• 通讯作者: Noriko Yoshihara

Pigment components in the flowers of Dutch iris cultivars and their contribution to the flower color

荷兰鸢尾品种花朵中的色素成分及其对花色的贡献

• 发表时间: 2011
• 作者: Mizuno Takayuki;Yabuya Tsutomu;Kitajima Junichi;Iwashina Tsukasa
• 通讯作者: Iwashina Tsukasa

Comparison of flower color and flavonoid composition between blues and violet flowers of Dutch iris cultivars

荷兰鸢尾品种蓝色和紫色花的花色和黄酮类成分比较

• 发表时间: 2012
• 作者: 藪谷 勤;花崎 歩;冨田武志;井上公一;Takayuki Mizuno
• 通讯作者: Takayuki Mizuno